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Borate Buffer Preparation and Recipe

Borate buffer is a widely use buffer with many applications. Borate buffers that contain sodium chloride have been used to study corrosion.

To prepare  L of Borate Buffer:
Change the value in the textbox above to scale the recipe volume

Table 1. Required components
ComponentAmountConcentration
Boric acid (mw: 61.83 g/mol)6.185 g0.1 M
Sodium chloride (mw: 58.44 g/mol)4.385 g0.075 M
Sodium tetraborate (mw: 201.22 g/mol)5.031 g0.025 M
  1. Prepare 800 mL of distilled water in a suitable container.
  2. Add 6.185 g of Boric acid to the solution.
  3. Add 4.385 g of Sodium chloride to the solution.
  4. Add 5.031 g of Sodium tetraborate to the solution.
  5. Add distilled water until the volume is 1 L.
  6. Use a stir bar and low heat to ensure that the boric acid dissolves fully. Sterilize by vacuum-filtration with a 0.22-µm filter. Adjust the pH to 8.4.

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Physiological Buffer
pH Buffering
Sample Preparation
BioAssays
Misc
Cell/Culture/Growth Media
Gel Electrophoresis


References

This online tool may be cited as follows

MLA

"Quest Calculate™ Borate Buffer Preparation and Recipe." AAT Bioquest, Inc.7 Dec2022https://www.aatbio.com/resources/buffer-preparations-and-recipes/borate-buffer.

APA

AAT Bioquest, Inc. (2022December 7). Quest Calculate™ Borate Buffer Preparation and Recipe. AAT Bioquest. https://www.aatbio.com/resources/buffer-preparations-and-recipes/borate-buffer.