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Borate Buffer Preparation and Recipe
Borate buffer is a widely use buffer with many applications. Borate buffers that contain sodium chloride have been used to study corrosion.
To prepare
L of Borate Buffer:
Change the value in the textbox above to scale the recipe volume
Table 1. Required components
ComponentAmountConcentration
Boric acid (mw: 61.83 g / mol)##
Sodium chloride (mw: 58.44 g / mol)##
Sodium tetraborate (mw: 201.22 g / mol)##
  • Prepare 800 mL of distilled water in a suitable container.
  • Add # of Boric acid to the solution
  • Add # of Sodium chloride to the solution
  • Add # of Sodium tetraborate to the solution
  • Add distilled water until the volume is 1 L.
  • Use a stir bar and low heat to ensure that the boric acid dissolves fully. Sterilize by vacuum-filtration with a 0.22-µm filter. Adjust the pH to 8.4.
References
This online resource may be cited as follows
MLA
"Quest Calculate™ Borate Buffer Preparation and Recipe." AAT Bioquest, Inc., 25 Oct. 2025, https://www.aatbio.com/resources/buffer-preparations-and-recipes/borate-buffer.
APA
AAT Bioquest, Inc. (2025, October 25). Quest Calculate™ Borate Buffer Preparation and Recipe. AAT Bioquest. https://www.aatbio.com/resources/buffer-preparations-and-recipes/borate-buffer.
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