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Embryonic Medium Preparation and Recipe

Embryonic medium is used for culturing embryos.

To prepare  L of Embryonic Medium:
Change the value in the textbox above to scale the recipe volume

Table 1. Required components
ComponentAmountConcentration
Sodium chloride (mw: 58.44 g/mol)0.87 g0.015 M
Potassium Chloride (mw: 74.55 g/mol)0.04 g0.0005 M
Calcium chloride dihydrate (mw: 147.01 g/mol)0.15 g0.001 M
Magnesium Sulfate Heptahydrate (mw: 246.47 g/mol)0.25 g0.001 M
Potassium Phosphate Monobasic (mw: 136.09 g/mol)0.02 g0.00015 M
Sodium phosphate dibaic dodecahydrate (mw: 358.14 g/mol)0.02 g0.00005 M
Sodium bicarbonate (mw: 84.01 g/mol)0.06 g0.0007 M
  1. Prepare 800 mL of distilled water in a suitable container.
  2. Add 0.87 g of Sodium chloride to the solution.
  3. Add 0.04 g of Potassium Chloride to the solution.
  4. Add 0.15 g of Calcium chloride dihydrate to the solution.
  5. Add 0.25 g of Magnesium Sulfate Heptahydrate to the solution.
  6. Add 0.02 g of Potassium Phosphate Monobasic to the solution.
  7. Add 0.02 g of Sodium phosphate dibaic dodecahydrate to the solution.
  8. Add 0.06 g of Sodium bicarbonate to the solution.
  9. Add distilled water until the volume is 1 L.
  10. Adjust the pH to 7.0-7.5.

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Physiological Buffer
pH Buffering
Sample Preparation
BioAssays
Misc
Cell/Culture/Growth Media
Gel Electrophoresis


References

This online tool may be cited as follows

MLA

"Quest Calculate™ Embryonic Medium Preparation and Recipe." AAT Bioquest, Inc.1 Oct2022https://www.aatbio.com/resources/buffer-preparations-and-recipes/embryonic-medium.

APA

AAT Bioquest, Inc. (2022October 1). Quest Calculate™ Embryonic Medium Preparation and Recipe. AAT Bioquest. https://www.aatbio.com/resources/buffer-preparations-and-recipes/embryonic-medium.