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Embryonic Medium Preparation and Recipe
Embryonic medium is used for culturing embryos.
To prepareL of Embryonic Medium:
Change the value in the textbox above to scale the recipe volume
Table 1. Required components
| Component | Amount | Concentration |
|---|---|---|
| Sodium chloride (mw: 58.44 g / mol) | # | # |
| Potassium Chloride (mw: 74.55 g / mol) | # | # |
| Calcium chloride dihydrate (mw: 147.01 g / mol) | # | # |
| Magnesium Sulfate Heptahydrate (mw: 246.47 g / mol) | # | # |
| Potassium Phosphate Monobasic (mw: 136.09 g / mol) | # | # |
| Sodium phosphate dibaic dodecahydrate (mw: 358.14 g / mol) | # | # |
| Sodium bicarbonate (mw: 84.01 g / mol) | # | # |
- Prepare 800 mL of distilled water in a suitable container.
- Add # of Sodium chloride to the solution
- Add # of Potassium Chloride to the solution
- Add # of Calcium chloride dihydrate to the solution
- Add # of Magnesium Sulfate Heptahydrate to the solution
- Add # of Potassium Phosphate Monobasic to the solution
- Add # of Sodium phosphate dibaic dodecahydrate to the solution
- Add # of Sodium bicarbonate to the solution
- Add distilled water until the volume is 1 L.
- Adjust the pH to 7.0-7.5.
References
This online resource may be cited as follows
MLA | "Quest Calculate™ Embryonic Medium Preparation and Recipe." AAT Bioquest, Inc., 23 Oct. 2025, https://www.aatbio.com/resources/buffer-preparations-and-recipes/embryonic-medium. | |
APA | AAT Bioquest, Inc. (2025, October 23). Quest Calculate™ Embryonic Medium Preparation and Recipe. AAT Bioquest. https://www.aatbio.com/resources/buffer-preparations-and-recipes/embryonic-medium. | |
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