Embryonic Medium Preparation and Recipe

Table 1. Required components

Component	Amount	Concentration
Sodium chloride (mw: 58.44 g/mol)	0.87 g	0.015 M
Potassium Chloride (mw: 74.55 g/mol)	0.04 g	0.0005 M
Calcium chloride dihydrate (mw: 147.01 g/mol)	0.15 g	0.001 M
Magnesium Sulfate Heptahydrate (mw: 246.47 g/mol)	0.25 g	0.001 M
Potassium Phosphate Monobasic (mw: 136.09 g/mol)	0.02 g	0.00015 M
Sodium phosphate dibaic dodecahydrate (mw: 358.14 g/mol)	0.02 g	0.00005 M
Sodium bicarbonate (mw: 84.01 g/mol)	0.06 g	0.0007 M

1. Prepare 800 mL of distilled water in a suitable container.
2. Add 0.87 g of Sodium chloride to the solution.
3. Add 0.04 g of Potassium Chloride to the solution.
4. Add 0.15 g of Calcium chloride dihydrate to the solution.
5. Add 0.25 g of Magnesium Sulfate Heptahydrate to the solution.
6. Add 0.02 g of Potassium Phosphate Monobasic to the solution.
7. Add 0.02 g of Sodium phosphate dibaic dodecahydrate to the solution.
8. Add 0.06 g of Sodium bicarbonate to the solution.
9. Add distilled water until the volume is 1 L.
10. Adjust the pH to 7.0-7.5.

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