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Sodium Carbonate Transfer Buffer (40x, pH 9.5) Preparation and Recipe
Sodium Carbonate Transfer Buffer is used to transfer proteins following SDS-PAGE.
To prepareL of Sodium Carbonate Transfer Buffer (40x, pH 9.5):
Change the value in the textbox above to scale the recipe volume
Table 1. Required components
| Component | Amount | Concentration |
|---|---|---|
| Sodium bicarbonate (mw: 84.01 g / mol) | # | # |
| Sodium carbonate (mw: 105.99 g / mol) | # | # |
- Prepare 800 mL of distilled water in a suitable container.
- Add # of Sodium bicarbonate to the solution
- Add # of Sodium carbonate to the solution
- Adjust the pH to 9.5 with 10 N NaOH.
- Add distilled water until the volume is 1 L.
- Store at room temperature.
References
This online resource may be cited as follows
MLA | "Quest Calculate™ Sodium Carbonate Transfer Buffer (40x, pH 9.5) Preparation and Recipe." AAT Bioquest, Inc., 28 Oct. 2025, https://www.aatbio.com/resources/buffer-preparations-and-recipes/sodium-carbonate-transfer-buffer-40x-ph-9-5. | |
APA | AAT Bioquest, Inc. (2025, October 28). Quest Calculate™ Sodium Carbonate Transfer Buffer (40x, pH 9.5) Preparation and Recipe. AAT Bioquest. https://www.aatbio.com/resources/buffer-preparations-and-recipes/sodium-carbonate-transfer-buffer-40x-ph-9-5. | |
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