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Sodium Carbonate Transfer Buffer (40x, pH 9.5) Preparation and Recipe

Sodium Carbonate Transfer Buffer is used to transfer proteins following SDS-PAGE.

To prepare  L of Sodium Carbonate Transfer Buffer (40x, pH 9.5):
Change the value in the textbox above to scale the recipe volume

Table 1. Required components
Sodium bicarbonate (mw: 84.01 g/mol)21.1 g0.251 M
Sodium carbonate (mw: 105.99 g/mol)18.35 g0.173 M
  1. Prepare 800 mL of distilled water in a suitable container.
  2. Add 21.1 g of Sodium bicarbonate to the solution.
  3. Add 18.35 g of Sodium carbonate to the solution.
  4. Adjust the pH to 9.5 with 10 N NaOH.
  5. Add distilled water until the volume is 1 L.
  6. Store at room temperature.

Physiological Buffer
pH Buffering
Sample Preparation
Cell/Culture/Growth Media
Gel Electrophoresis


This online tool may be cited as follows


"Quest Calculate™ Sodium Carbonate Transfer Buffer (40x, pH 9.5) Preparation and Recipe." AAT Bioquest, Inc.12 Jul2024https://www.aatbio.com/resources/buffer-preparations-and-recipes/sodium-carbonate-transfer-buffer-40x-ph-9-5.


AAT Bioquest, Inc. (2024July 12). Quest Calculate™ Sodium Carbonate Transfer Buffer (40x, pH 9.5) Preparation and Recipe. AAT Bioquest. https://www.aatbio.com/resources/buffer-preparations-and-recipes/sodium-carbonate-transfer-buffer-40x-ph-9-5.