Actively helping customers, employees and the global community during the coronavirus SARS-CoV-2 outbreak.  Learn more >>

Sodium Carbonate Transfer Buffer (40x, pH 9.5) Preparation and Recipe

Sodium Carbonate Transfer Buffer is used to transfer proteins following SDS-PAGE.

To prepare  L of Sodium Carbonate Transfer Buffer (40x, pH 9.5):
Change the value in the textbox above to scale the recipe volume

Table 1. Required components
ComponentAmountConcentration
Sodium bicarbonate (mw: 84.01 g/mol)21.1 g0.251 M
Sodium carbonate (mw: 105.99 g/mol)18.35 g0.173 M
  1. Prepare 800 mL of distilled water in a suitable container.
  2. Add 21.1 g of Sodium bicarbonate to the solution.
  3. Add 18.35 g of Sodium carbonate to the solution.
  4. Adjust the pH to 9.5 with 10 N NaOH.
  5. Add distilled water until the volume is 1 L.
  6. Store at room temperature.

To make a purchase inquiry for this buffer, please provide your email address below:
Request quotation



Physiological Buffer
pH Buffering
Sample Preparation
BioAssays
Misc
Cell/Culture/Growth Media
Gel Electrophoresis


References

This online tool may be cited as follows

MLA

"Quest Calculate™ Sodium Carbonate Transfer Buffer (40x, pH 9.5) Preparation and Recipe." AAT Bioquest, Inc.1 Oct2022https://www.aatbio.com/resources/buffer-preparations-and-recipes/sodium-carbonate-transfer-buffer-40x-ph-9-5.

APA

AAT Bioquest, Inc. (2022October 1). Quest Calculate™ Sodium Carbonate Transfer Buffer (40x, pH 9.5) Preparation and Recipe. AAT Bioquest. https://www.aatbio.com/resources/buffer-preparations-and-recipes/sodium-carbonate-transfer-buffer-40x-ph-9-5.