Imidazole-HCl Buffer (0.05 M, pH 7.0) Preparation and Recipe

Imidazole is a mild buffer that is useful for IHC applications in multiple research areas such as biochemistry and molecular biology. It has a good shelf-life and is well-tolerated by multiple cell types.

To prepare

L of Imidazole-HCl Buffer (0.05 M, pH 7.0):

Change the value in the textbox above to scale the recipe volume

Table 1. Required components

Component	Amount	Concentration
Imidazole (mw: 68.08 g / mol)	#	#
Hydrochloric acid (mw: 36.46 g / mol)	#	#

Prepare 800 mL of distilled water in a suitable container.
Add # of Imidazole to the solution
Add # of Hydrochloric acid to the solution
Adjust solution to desired pH using HCl (typically pH ≈ 7.0).
Add distilled water until the volume is 1 L.

References

This online resource may be cited as follows

MLA	"Quest Calculate™ Imidazole-HCl Buffer (0.05 M, pH 7.0) Preparation and Recipe." AAT Bioquest, Inc., 14 Oct. 2025, https://www.aatbio.com/resources/buffer-preparations-and-recipes/imidazole-hcl-buffer-ph-7-0.
APA	AAT Bioquest, Inc. (2025, October 14). Quest Calculate™ Imidazole-HCl Buffer (0.05 M, pH 7.0) Preparation and Recipe. AAT Bioquest. https://www.aatbio.com/resources/buffer-preparations-and-recipes/imidazole-hcl-buffer-ph-7-0.
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