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Imidazole-HCl Buffer (0.05 M, pH 7.0) Preparation and Recipe

Imidazole is a mild buffer that is useful for IHC applications in multiple research areas such as biochemistry and molecular biology. It has a good shelf-life and is well-tolerated by multiple cell types.

To prepare  L of Imidazole-HCl Buffer (0.05 M, pH 7.0):
Change the value in the textbox above to scale the recipe volume

Table 1. Required components
Imidazole (mw: 68.08 g/mol)3.404 g0.05 M
Hydrochloric acid (mw: 36.46 g/mol)0.886 g0.0243 M
  1. Prepare 800 mL of distilled water in a suitable container.
  2. Add 3.404 g of Imidazole to the solution.
  3. Add 0.886 g of Hydrochloric acid to the solution.
  4. Adjust solution to desired pH using HCl (typically pH ≈ 7.0).
  5. Add distilled water until the volume is 1 L.

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Physiological Buffer
pH Buffering
Sample Preparation
Cell/Culture/Growth Media
Gel Electrophoresis


This online tool may be cited as follows


"Quest Calculate™ Imidazole-HCl Buffer (0.05 M, pH 7.0) Preparation and Recipe." AAT Bioquest, Inc.1 Oct2022


AAT Bioquest, Inc. (2022October 1). Quest Calculate™ Imidazole-HCl Buffer (0.05 M, pH 7.0) Preparation and Recipe. AAT Bioquest.