AAT Bioquest

SSC (Northern Blot, 20x) Preparation and Recipe

SSC (saline sodium citrate) buffer is commonly used in Northern blotting as a hybridization or wash buffer.

To prepare  L of SSC (Northern Blot, 20x):
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Table 1. Required components
Sodium chloride (mw: 58.44 g/mol)175.3 g3 M
Trisodium citrate (mw: 258.07 g/mol)77.4 g0.3 M
  1. Prepare 800 mL of distilled water in a suitable container.
  2. Add 175.3 g of Sodium chloride to the solution.
  3. Add 77.4 g of Trisodium citrate to the solution.
  4. Add distilled water until the volume is 1 L.
  5. Adjust the pH to 7.0 with NaOH or HCl. Autoclave the solution and store it at room temperature. It will last for 1 yr or longer.

Physiological Buffer
pH Buffering
Sample Preparation
Cell/Culture/Growth Media
Gel Electrophoresis


This online tool may be cited as follows


"Quest Calculate™ SSC (Northern Blot, 20x) Preparation and Recipe." AAT Bioquest, Inc.23 Jul2024https://www.aatbio.com/resources/buffer-preparations-and-recipes/ssc-for-northerns-20x.


AAT Bioquest, Inc. (2024July 23). Quest Calculate™ SSC (Northern Blot, 20x) Preparation and Recipe. AAT Bioquest. https://www.aatbio.com/resources/buffer-preparations-and-recipes/ssc-for-northerns-20x.