AAT Bioquest

CAPS (0.5 M, pH 10.4) Preparation and Recipe

CAPS is used commonly for Western Blot applications and other protein sorting procedures. The very high pH of this buffer allows for identification and sequencing of peptides and proteins with high isoelectric points (pl or IEP).

To prepare  L of CAPS (0.5 M, pH 10.4):
Change the value in the textbox above to scale the recipe volume

Table 1. Required components
CAPS (mw: 221.32 g/mol)110.66 g0.5 M
  1. Prepare 800 mL of dH2O in a suitable container.
  2. Add 110.66 g of CAPS to the solution.
  3. Adjust solution to desired pH using 10N NaOH.
  4. Add dH2O until the volume is 1 L.

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Physiological Buffer
pH Buffering
Sample Preparation
Cell/Culture/Growth Media
Gel Electrophoresis


This online tool may be cited as follows


"Quest Calculate™ CAPS (0.5 M, pH 10.4) Preparation and Recipe." AAT Bioquest, Inc.29 May2024https://www.aatbio.com/resources/buffer-preparations-and-recipes/caps-ph-10-4.


AAT Bioquest, Inc. (2024May 29). Quest Calculate™ CAPS (0.5 M, pH 10.4) Preparation and Recipe. AAT Bioquest. https://www.aatbio.com/resources/buffer-preparations-and-recipes/caps-ph-10-4.