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CAPS (0.5 M, pH 10.4) Preparation and Recipe

CAPS is used commonly for Western Blot applications and other protein sorting procedures. The very high pH of this buffer allows for identification and sequencing of peptides and proteins with high isoelectric points (pl or IEP).

To prepare  L of CAPS (0.5 M, pH 10.4):
Change the value in the textbox above to scale the recipe volume

Table 1. Required components
ComponentAmountConcentration
CAPS (mw: 221.32 g/mol)110.66 g0.5 M
  1. Prepare 800 mL of dH2O in a suitable container.
  2. Add 110.66 g of CAPS to the solution.
  3. Adjust solution to desired pH using 10N NaOH.
  4. Add dH2O until the volume is 1 L.

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Physiological Buffer
pH Buffering
Sample Preparation
BioAssays
Misc
Cell/Culture/Growth Media
Gel Electrophoresis


References

This online tool may be cited as follows

MLA

"Quest Calculate™ CAPS (0.5 M, pH 10.4) Preparation and Recipe." AAT Bioquest, Inc.6 Dec2022https://www.aatbio.com/resources/buffer-preparations-and-recipes/caps-ph-10-4.

APA

AAT Bioquest, Inc. (2022December 6). Quest Calculate™ CAPS (0.5 M, pH 10.4) Preparation and Recipe. AAT Bioquest. https://www.aatbio.com/resources/buffer-preparations-and-recipes/caps-ph-10-4.