CAPS (0.5 M, pH 10.4) Preparation and Recipe
CAPS is used commonly for Western Blot applications and other protein sorting procedures. The very high pH of this buffer allows for identification and sequencing of peptides and proteins with high isoelectric points (pl or IEP).
To prepare L of CAPS (0.5 M, pH 10.4):
Change the value in the textbox above to scale the recipe volume
Change the value in the textbox above to scale the recipe volume
Table 1. Required components
Component | Amount | Concentration |
CAPS (mw: 221.32 g/mol) | 110.66 g | 0.5 M |
- Prepare 800 mL of dH2O in a suitable container.
- Add 110.66 g of CAPS to the solution.
- Adjust solution to desired pH using 10N NaOH.
- Add dH2O until the volume is 1 L.
Physiological Buffer
pH Buffering
Sample Preparation
BioAssays
Misc
Cell/Culture/Growth Media
Gel Electrophoresis
References
This online tool may be cited as follows
MLA | "Quest Calculate™ CAPS (0.5 M, pH 10.4) Preparation and Recipe." AAT Bioquest, Inc., 14 Nov. 2024, https://www.aatbio.com/resources/buffer-preparations-and-recipes/caps-ph-10-4. | |
APA | AAT Bioquest, Inc. (2024, November 14). Quest Calculate™ CAPS (0.5 M, pH 10.4) Preparation and Recipe. AAT Bioquest. https://www.aatbio.com/resources/buffer-preparations-and-recipes/caps-ph-10-4. | |
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