CAPS (0.5 M, pH 10.4) Preparation and Recipe

CAPS is used commonly for Western Blot applications and other protein sorting procedures. The very high pH of this buffer allows for identification and sequencing of peptides and proteins with high isoelectric points (pl or IEP).

To prepare

L of CAPS (0.5 M, pH 10.4):

Change the value in the textbox above to scale the recipe volume

Table 1. Required components

Component	Amount	Concentration
CAPS (mw: 221.32 g / mol)	#	#

Prepare 800 mL of dH2O in a suitable container.
Add # of CAPS to the solution
Adjust solution to desired pH using 10N NaOH.
Add dH2O until the volume is 1 L.

References

This online resource may be cited as follows

MLA	"Quest Calculate™ CAPS (0.5 M, pH 10.4) Preparation and Recipe." AAT Bioquest, Inc., 13 Oct. 2025, https://www.aatbio.com/resources/buffer-preparations-and-recipes/caps-ph-10-4.
APA	AAT Bioquest, Inc. (2025, October 13). Quest Calculate™ CAPS (0.5 M, pH 10.4) Preparation and Recipe. AAT Bioquest. https://www.aatbio.com/resources/buffer-preparations-and-recipes/caps-ph-10-4.
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