MES (0.5 M, pH 6) Preparation and Recipe

MES buffer is a Good's buffer that is remarkably stable both chemically and enzymatically. It has minimal UV absorbance and is often used as a running buffer for bis-tris gels.

To prepare

L of MES (0.5 M, pH 6):

Change the value in the textbox above to scale the recipe volume

Table 1. Required components

Component	Amount	Concentration
MES free acid (mw: 195.24 g / mol)	#	#

Prepare 800 mL of dH2O in a suitable container.
Add # of MES free acid to the solution
Starting pH for 0.5M MES solution is 3.23 For 1 L of 0.5 M MES, 13.6 ml of 10N NaOH is needed to adjust the pH to 6.0
Add dH2O until the volume is 1 L.

References

This online resource may be cited as follows

MLA	"Quest Calculate™ MES (0.5 M, pH 6) Preparation and Recipe." AAT Bioquest, Inc., 7 Oct. 2025, https://www.aatbio.com/resources/buffer-preparations-and-recipes/mes-ph-6.
APA	AAT Bioquest, Inc. (2025, October 7). Quest Calculate™ MES (0.5 M, pH 6) Preparation and Recipe. AAT Bioquest. https://www.aatbio.com/resources/buffer-preparations-and-recipes/mes-ph-6.
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