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MES (0.5 M, pH 6) Preparation and Recipe

MES buffer is a Good's buffer that is remarkably stable both chemically and enzymatically. It has minimal UV absorbance and is often used as a running buffer for bis-tris gels.

To prepare  L of MES (0.5 M, pH 6):
Change the value in the textbox above to scale the recipe volume

Table 1. Required components
ComponentAmountConcentration
MES free acid (mw: 195.24 g/mol)97.62 g0.5 M
  1. Prepare 800 mL of dH2O in a suitable container.
  2. Add 97.62 g of MES free acid to the solution.
  3. Starting pH for 0.5M MES solution is 3.23 For 1 L of 0.5 M MES, 13.6 ml of 10N NaOH is needed to adjust the pH to 6.0
  4. Add dH2O until the volume is 1 L.

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Physiological Buffer
pH Buffering
Sample Preparation
BioAssays
Misc
Cell/Culture/Growth Media
Gel Electrophoresis


References

This online tool may be cited as follows

MLA

"Quest Calculate™ MES (0.5 M, pH 6) Preparation and Recipe." AAT Bioquest, Inc.1 Oct2022https://www.aatbio.com/resources/buffer-preparations-and-recipes/mes-ph-6.

APA

AAT Bioquest, Inc. (2022October 1). Quest Calculate™ MES (0.5 M, pH 6) Preparation and Recipe. AAT Bioquest. https://www.aatbio.com/resources/buffer-preparations-and-recipes/mes-ph-6.