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MES (0.5 M, pH 6) Preparation and Recipe

MES buffer is a Good's buffer that is remarkably stable both chemically and enzymatically. It has minimal UV absorbance and is often used as a running buffer for bis-tris gels.

To prepare  L of MES (0.5 M, pH 6):
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Table 1. Required components
MES free acid (mw: 195.24 g/mol)97.62 g0.5 M
  1. Prepare 800 mL of dH2O in a suitable container.
  2. Add 97.62 g of MES free acid to the solution.
  3. Starting pH for 0.5M MES solution is 3.23 For 1 L of 0.5 M MES, 13.6 ml of 10N NaOH is needed to adjust the pH to 6.0
  4. Add dH2O until the volume is 1 L.

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Physiological Buffer
pH Buffering
Sample Preparation
Cell/Culture/Growth Media
Gel Electrophoresis


This online tool may be cited as follows


"Quest Calculate™ MES (0.5 M, pH 6) Preparation and Recipe." AAT Bioquest, Inc.23 Jun2024https://www.aatbio.com/resources/buffer-preparations-and-recipes/mes-ph-6.


AAT Bioquest, Inc. (2024June 23). Quest Calculate™ MES (0.5 M, pH 6) Preparation and Recipe. AAT Bioquest. https://www.aatbio.com/resources/buffer-preparations-and-recipes/mes-ph-6.