MES (0.5 M, pH 6) recipe and preparation

MES buffer was developed as one of the Good's buffer that is chemically and enzymatically stable. It has minimal UV absorbance and it is often used as a running buffer for bis-tris gels

To prepare L of MES (0.5 M, pH 6):
Change the value in the textbox above to scale the recipe volume

Table 1. Required components
ComponentMassMolarity
MES free acid (mw: 195 g/mol)97.62 g0.5 M
  1. Prepare 800 mL of dH2O in a suitable container.
  2. Add 97.62 g of MES free acid to the solution.
  3. Starting pH for 0.5M MES solution is 3.23 For 1 L of 0.5 M MES, 13.6 ml of 10N NaOH is needed to adjust the pH to 6.0
  4. Add distilled water until volume is 1 L.
 

Buffer

Min pH

Max pH

Type

Acetate Buffer (0.1 M, pH 5.0)3.75.6Common Physiological
BES-Buffered Saline (2X) (0.05 M, pH 6.95)6.47.8Common Physiological
Bicine (1 M, pH 8.26)7.69.0Common Physiological
CAPS (0.5 M, pH 10.4)9.711.1Common Physiological
CHES (0.5 M, pH 9.5)8.610.0Common Physiological
Citrate Buffer (0.1 M, pH 6.0)3.06.2Common Physiological
Citrate-Phosphate Buffer (0.15 M, pH 5.0)2.67.6Common Physiological
Diethanolamine (1 M, pH 9.8)7.89.9Common Physiological
EBSS (magnesium, calcium, phenol red) (pH 7.0)7.07.4Common Physiological
Glycine-HCl Buffer (0.1 M, pH 3.0)2.23.6Common Physiological
Glycine-Sodium Hydroxide Buffer (0.08 M, pH 10)8.610.6Common Physiological
HBSS (Hank's Balanced Salt Solution)7.27.6Common Physiological
HEPPSO (1 M, pH 7.85)7.18.5Common Physiological
HHBS (Hank's Buffer with Hepes)7.27.4Common Physiological
Hydrochloric Acid-Potassium Chloride Buffer (0.1 M, pH 2.0)1.02.2Common Physiological
Imidazole-HCl Buffer (0.05 M, pH 7.0)6.27.8Common Physiological
Maleic Acid (1 M, pH 7.5)7.08.0Common Physiological
MES (0.5 M, pH 6)5.56.7Common Physiological
MOPS Buffer (10X) (0.2 M, pH 7)6.57.9Common Physiological
PBS (Phosphate Buffered Saline) (1X, pH 7.4)7.37.5Common Physiological
Sodium Borate Buffer (1 M, pH 8.5)8.010.0Common Physiological
TAE (1 M, pH 8.6)8.08.5Common Physiological
TAE Buffer (50X) (0.04 M, pH 8.5)8.08.5Common Physiological
TBS (1 M, pH 7.4)7.27.5Common Physiological
TE Buffer 10X7.58.5Common Physiological
Tricine (1 M, pH 8.05)7.48.8Common Physiological
Tris Buffer (1 M, pH 7.0)7.07.5Common Physiological
Acetate Buffer (pH 3.6 to 5.6)3.75.6Variable pH
Carbonate-Bicarbonate Buffer (pH 9.2 to 10.6)9.210.8Variable pH
Citrate Buffer (pH 3.0 to 6.2)3.06.2Variable pH
Phosphate Buffer (pH 5.8 to 7.4)5.88.0Variable pH
Potassium Phosphate (pH 5.8 to 8.0)5.88.0Variable pH
Trizma Buffer (pH 7.2 to 9.0)7.29.0Variable pH