Actively helping customers, employees and the global community during the coronavirus SARS-CoV-2 outbreak.  Learn more >>

SSC buffer (20X) (3 M, pH 7) Preparation and Recipe

Saline-sodium citrate (SSC) buffer is often used as a hybridization buffer for DNA and RNA, particularly for Northern and Southern blots.

To prepare  L of SSC buffer (20X) (3 M, pH 7):
Change the value in the textbox above to scale the recipe volume

Table 1. Required components
ComponentAmountConcentration
Sodium chloride (mw: 58.44 g/mol)175.3 g3 M
Sodium citrate (mw: 294.10 g/mol)77.4 g0.3 M
  1. Prepare 800 mL of dH2O in a suitable container.
  2. Add 175.3 g of Sodium chloride to the solution.
  3. Add 77.4 g of Sodium citrate to the solution.
  4. Adjust solution to desired pH with 14 N HCl (typically 7.0).
  5. Add dH2O until the volume is 1 L.

To make a purchase inquiry for this buffer, please provide your email address below:
Request quotation



Physiological Buffer
pH Buffering
Sample Preparation
BioAssays
Misc
Cell/Culture/Growth Media
Gel Electrophoresis


References

This online tool may be cited as follows

MLA

"Quest Calculate™ SSC buffer (20X) (3 M, pH 7) Preparation and Recipe." AAT Bioquest, Inc.6 Dec2022https://www.aatbio.com/resources/buffer-preparations-and-recipes/ssc-buffer-20x-ph-7.

APA

AAT Bioquest, Inc. (2022December 6). Quest Calculate™ SSC buffer (20X) (3 M, pH 7) Preparation and Recipe. AAT Bioquest. https://www.aatbio.com/resources/buffer-preparations-and-recipes/ssc-buffer-20x-ph-7.