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SSC buffer (20X) (3 M, pH 7) Preparation and Recipe
Saline-sodium citrate (SSC) buffer is often used as a hybridization buffer for DNA and RNA, particularly for Northern and Southern blots.
To prepareL of SSC buffer (20X) (3 M, pH 7):
Change the value in the textbox above to scale the recipe volume
Table 1. Required components
| Component | Amount | Concentration |
|---|---|---|
| Sodium chloride (mw: 58.44 g / mol) | # | # |
| Sodium citrate (mw: 294.1 g / mol) | # | # |
- Prepare 800 mL of dH2O in a suitable container.
- Add # of Sodium chloride to the solution
- Add # of Sodium citrate to the solution
- Adjust solution to desired pH with 14 N HCl (typically 7.0).
- Add dH2O until the volume is 1 L.
References
This online resource may be cited as follows
MLA | "Quest Calculate™ SSC buffer (20X) (3 M, pH 7) Preparation and Recipe." AAT Bioquest, Inc., 22 Dec. 2025, https://www.aatbio.com/resources/buffer-preparations-and-recipes/ssc-buffer-20x-ph-7. | |
APA | AAT Bioquest, Inc. (2025, December 22). Quest Calculate™ SSC buffer (20X) (3 M, pH 7) Preparation and Recipe. AAT Bioquest. https://www.aatbio.com/resources/buffer-preparations-and-recipes/ssc-buffer-20x-ph-7. | |
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