AAT Bioquest

SSC buffer (20X) (3 M, pH 7) Preparation and Recipe

Saline-sodium citrate (SSC) buffer is often used as a hybridization buffer for DNA and RNA, particularly for Northern and Southern blots.

To prepare  L of SSC buffer (20X) (3 M, pH 7):
Change the value in the textbox above to scale the recipe volume

Table 1. Required components
Sodium chloride (mw: 58.44 g/mol)175.3 g3 M
Sodium citrate (mw: 294.10 g/mol)88.3 g0.3 M
  1. Prepare 800 mL of dH2O in a suitable container.
  2. Add 175.3 g of Sodium chloride to the solution.
  3. Add 88.3 g of Sodium citrate to the solution.
  4. Adjust solution to desired pH with 14 N HCl (typically 7.0).
  5. Add dH2O until the volume is 1 L.

Physiological Buffer
pH Buffering
Sample Preparation
Cell/Culture/Growth Media
Gel Electrophoresis


This online tool may be cited as follows


"Quest Calculate™ SSC buffer (20X) (3 M, pH 7) Preparation and Recipe." AAT Bioquest, Inc.13 Jul2024https://www.aatbio.com/resources/buffer-preparations-and-recipes/ssc-buffer-20x-ph-7.


AAT Bioquest, Inc. (2024July 13). Quest Calculate™ SSC buffer (20X) (3 M, pH 7) Preparation and Recipe. AAT Bioquest. https://www.aatbio.com/resources/buffer-preparations-and-recipes/ssc-buffer-20x-ph-7.