AAT Bioquest

SSPE (Blot, 20x, pH 7.4) Preparation and Recipe

SSPE (saline sodium phosphate EDTA) is used in hybridization and bacterial screening.

To prepare  L of SSPE (Blot, 20x, pH 7.4):
Change the value in the textbox above to scale the recipe volume

Table 1. Required components
Sodium chloride (mw: 58.44 g/mol)175.3 g3 M
Sodium Phosphate Monobasic Monohydrate (mw: 137.99 g/mol)27.6 g0.2 M
EDTA (mw: 292.24 g/mol)7.4 g0.02 M
  1. Prepare 800 mL of distilled water in a suitable container.
  2. Add 175.3 g of Sodium chloride to the solution.
  3. Add 27.6 g of Sodium Phosphate Monobasic Monohydrate to the solution.
  4. Add 7.4 g of EDTA to the solution.
  5. Add distilled water until the volume is 1 L.
  6. Adjust the pH to 7.4 with 10 N NaOH. Store at room temperature.

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Physiological Buffer
pH Buffering
Sample Preparation
Cell/Culture/Growth Media
Gel Electrophoresis


This online tool may be cited as follows


"Quest Calculate™ SSPE (Blot, 20x, pH 7.4) Preparation and Recipe." AAT Bioquest, Inc.29 May2024https://www.aatbio.com/resources/buffer-preparations-and-recipes/sspe-20x-ph-7-4.


AAT Bioquest, Inc. (2024May 29). Quest Calculate™ SSPE (Blot, 20x, pH 7.4) Preparation and Recipe. AAT Bioquest. https://www.aatbio.com/resources/buffer-preparations-and-recipes/sspe-20x-ph-7-4.