SSPE (Blot, 20x, pH 7.4) Preparation and Recipe
SSPE (saline sodium phosphate EDTA) is used in hybridization and bacterial screening.
To prepare L of SSPE (Blot, 20x, pH 7.4):
Change the value in the textbox above to scale the recipe volume
Change the value in the textbox above to scale the recipe volume
Table 1. Required components
Component | Amount | Concentration |
Sodium chloride (mw: 58.44 g/mol) | 175.3 g | 3 M |
Sodium Phosphate Monobasic Monohydrate (mw: 137.99 g/mol) | 27.6 g | 0.2 M |
EDTA (mw: 292.24 g/mol) | 7.4 g | 0.02 M |
- Prepare 800 mL of distilled water in a suitable container.
- Add 175.3 g of Sodium chloride to the solution.
- Add 27.6 g of Sodium Phosphate Monobasic Monohydrate to the solution.
- Add 7.4 g of EDTA to the solution.
- Add distilled water until the volume is 1 L.
- Adjust the pH to 7.4 with 10 N NaOH. Store at room temperature.
Physiological Buffer
pH Buffering
Sample Preparation
BioAssays
Misc
Cell/Culture/Growth Media
Gel Electrophoresis
References
This online tool may be cited as follows
MLA | "Quest Calculate™ SSPE (Blot, 20x, pH 7.4) Preparation and Recipe." AAT Bioquest, Inc., 12 Oct. 2024, https://www.aatbio.com/resources/buffer-preparations-and-recipes/sspe-20x-ph-7-4. | |
APA | AAT Bioquest, Inc. (2024, October 12). Quest Calculate™ SSPE (Blot, 20x, pH 7.4) Preparation and Recipe. AAT Bioquest. https://www.aatbio.com/resources/buffer-preparations-and-recipes/sspe-20x-ph-7-4. | |
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