PBS (Phosphate Buffered Saline) (1X, pH 7.4) recipe and preparation
PBS is a buffer frequently used in biological applications, such as washing cells, transportation of tissues, and dilutions. PBS closely mimics the pH, osmolarity, and ion concentrations of the human body.
To prepare L of PBS (Phosphate Buffered Saline) (1X, pH 7.4): Change the value in the textbox above to scale the recipe volume
Table 1. Required components
NaCl (mw: 58.4 g/mol)
KCl (mw: 74.551 g/mol)
Na2HPO4 (mw: 141.96 g/mol)
KH2PO4 (mw: 136.086 g/mol)
Prepare 800 mL of distilled water in a suitable container.
Add 8 g of NaCl to the solution.
Add 200 mg of KCl to the solution.
Add 1.44 g of Na2HPO4 to the solution.
Add 240 mg of KH2PO4 to the solution.
Adjust solution to desired pH (typically pH ≈ 7.4).