PBS (Phosphate Buffered Saline) (1X, pH 7.4) Preparation and Recipe

PBS is an isotonic buffer frequently used in biological applications, such as washing cells, transportation of tissues, and dilutions. PBS closely mimics the pH, osmolarity, and ion concentrations of the human body. Since it is nontoxic to cells, it is extensively used for cell container rinsing and other preparations that might leave a residue. It is simple to prepare and has good shelf life, but will precipitate in the presence of zinc ions.

To prepare L of PBS (Phosphate Buffered Saline) (1X, pH 7.4):
Change the value in the textbox above to scale the recipe volume

Table 1. Required components

Component	Amount	Concentration
Sodium chloride (mw: 58.44 g/mol)	8 g	0.137 M
Potassium Chloride (mw: 74.55 g/mol)	0.2 g	0.0027 M
Sodium Phosphate Dibasic (mw: 141.96 g/mol)	1.44 g	0.01 M
Potassium Phosphate Monobasic (mw: 136.09 g/mol)	0.245 g	0.0018 M

Prepare 800 mL of distilled water in a suitable container.
Add 8 g of Sodium chloride to the solution.
Add 0.2 g of Potassium Chloride to the solution.
Add 1.44 g of Sodium Phosphate Dibasic to the solution.
Add 0.245 g of Potassium Phosphate Monobasic to the solution.
Adjust solution to desired pH (typically pH ≈ 7.4).
Add distilled water until the volume is 1 L.

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Physiological Buffer

pH Buffering

Sample Preparation

BioAssays

Misc

Cell/Culture/Growth Media

Gel Electrophoresis

Name ▲ ▼
PBS (Phosphate Buffered Saline) (1X, pH 7.4)
HBSS (Hank's Balanced Salt Solution)
HHBS (Hank's Buffer with Hepes)
HEPES Buffer (1 M, 7.5 pH)
PBS with Ca2+and Mg2+ (D-PBS)
Extracellular Saline (1x, pH 7.3)
Tyrode's Solution, Acidic

Name ▲ ▼
Citrate Buffer (pH 3.0 to 6.2)
Acetate Buffer (pH 3.6 to 5.6)
Tris Buffer (1 M, pH 7.2)
Citrate Buffer (0.1 M, pH 6.0)
Acetate Buffer (0.1 M, pH 5.0)
Phosphate Buffer (pH 5.8 to 7.4)
Hydrochloric Acid-Potassium Chloride Buffer (0.1 M, pH 2.0)
Glycine-HCl Buffer (0.1 M, pH 3.0)
Citrate-Phosphate Buffer (0.15 M, pH 5.0)
Glycine-Sodium Hydroxide Buffer (0.08 M, pH 10)
EBSS (magnesium, calcium, phenol red) (pH 7.0)
Imidazole-HCl Buffer (0.05 M, pH 7.0)
Trizma® Buffer (pH 7.0 to 9.2)
MES (0.5 M, pH 6)
Bis-Tris Buffer (1 M, 6.5 pH)
ADA Buffer (0.5 M, 6.6 pH)
ACES Buffer (0.1 M, 6.7 pH)
PIPES Buffer (1 M, 6.8 pH)
MOPSO Buffer (0.5 M, 6.9 pH)
BES-Buffered Saline (2X) (0.05 M, pH 6.95)
TES Buffer (1 M, 7.5 pH)
MOPS Buffer (10X) (0.2 M, pH 7)
SSC buffer (20X) (3 M, pH 7)
TBS (1 M, pH 7.4)
Bicine (1 M, pH 8.26)
Tricine (1 M, pH 8.05)
TAE (1 M, pH 8.6)
CAPS (0.5 M, pH 10.4)
HEPPSO (1 M, pH 7.85)
CHES (0.5 M, pH 9.5)
Potassium Phosphate (pH 5.8 to 8.0)
Diethanolamine (1 M, pH 9.8)
Sodium Borate Buffer (1 M, pH 8.5)
Maleic Acid (1 M, pH 7.5)
Tris-Buffered Saline (TBS, 0.1 M)
Ammonium Sulfate, Saturated
Potassium Phosphate Buffer (1 M, pH 6.5)
Citrate-Phosphate Buffer (110 mM, pH 5.6)
Citrate-Phosphate Buffer (40 mM, pH 5.6)
Sodium Phosphate Solution
Sodium Acetate (3 M, pH 5.2)
TB Buffer
Tris-citrate Buffer (pH 8.0)
Borate Buffer
Glycine (0.1 M, pH 2.2)
Ammonium Bicarbonate (50 mM, pH 7.8)
Ammonium Acetate
Carbonate-Bicarbonate Buffer (pH 9.2 to 10.6)

Name ▲ ▼
TE Buffer 10X
Elution Buffer
BAC DNA Microinjection Buffer
EDTA Solution
ACK Lysis Buffer
Paraformaldehyde Solution (8%)
Gey's Balanced Salt Solution
Lysis Solution
H-50 Buffer
Alkaline Sodium Thiosulfate
Osmium Tetroxide Solution
DEAE-dextran
Trichloroacetic Acid (TCA)

Name ▲ ▼
Bradford Stock Solution
Anesthetic (MS222) Solution
5-Fluorocytosine (5-FC) Solution
SSC (Northern Blot, 20x)
SSPE (Blot, 20x, pH 7.4)
Immunofluorescence Quenching Solution
Nuclear Fast Red Solution
Pyronin Y (PY) Solution
Mayer's Hemalum
Chloroquine
Ethidium Bromide Solution
Cresol Red Solution

Name ▲ ▼
Sodium Iodide/Sodium Sulfite Solution
Dithiothreitol (DTT) (1 M)

Name ▲ ▼
Bath Solution (Ca2+/Mg2+ Free)
Modified Barth's Saline (MBS)
Calcium Chloride Stock Solution
Yeast Two Hybrid (Y2H) Media, Amino Acid Dropout Mixes
Digestion Medium
Chelation Medium
Penicillin/Streptomycin/Chloramphenicol Antibiotic Mix
Embryonic Medium
Carbenicillin Solution
M9 Minimal Salts
IPTG Solution

Name ▲ ▼
SDS-PAGE SDS Running Buffer (10x)
Electrophoretic Transfer Buffer (20x)
Tris-Glycine Transfer Buffer (20x)
Sodium Carbonate Transfer Buffer (40x, pH 9.5)
Acrylamide Stock Solution
Imidazole-SDS Solution
Electrode Buffer
Tris/EACA Buffer

References

This online tool may be cited as follows

MLA	"Quest Calculate™ PBS (Phosphate Buffered Saline) (1X, pH 7.4) Preparation and Recipe." AAT Bioquest, Inc., 26 Jan. 2023, https://www.aatbio.com/resources/buffer-preparations-and-recipes/pbs-phosphate-buffered-saline.
APA	AAT Bioquest, Inc. (2023, January 26). Quest Calculate™ PBS (Phosphate Buffered Saline) (1X, pH 7.4) Preparation and Recipe. AAT Bioquest. https://www.aatbio.com/resources/buffer-preparations-and-recipes/pbs-phosphate-buffered-saline.

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PBS (Phosphate Buffered Saline) (1X, pH 7.4) Preparation and Recipe

References

This online tool may be cited as follows

MLA

APA

This online tool has been cited in 5 publications, including