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PBS (Phosphate Buffered Saline) (1X, pH 7.4) Preparation and Recipe

PBS is an isotonic buffer frequently used in biological applications, such as washing cells, transportation of tissues, and dilutions. PBS closely mimics the pH, osmolarity, and ion concentrations of the human body. Since it is nontoxic to cells, it is extensively used for cell container rinsing and other preparations that might leave a residue. It is simple to prepare and has good shelf life, but will precipitate in the presence of zinc ions.

To prepare  L of PBS (Phosphate Buffered Saline) (1X, pH 7.4):
Change the value in the textbox above to scale the recipe volume

Table 1. Required components
Sodium chloride (mw: 58.44 g/mol)8 g0.137 M
Potassium Chloride (mw: 74.55 g/mol)0.2 g0.0027 M
Sodium Phosphate Dibasic (mw: 141.96 g/mol)1.44 g0.01 M
Potassium Phosphate Monobasic (mw: 136.09 g/mol)0.245 g0.0018 M
  1. Prepare 800 mL of distilled water in a suitable container.
  2. Add 8 g of Sodium chloride to the solution.
  3. Add 0.2 g of Potassium Chloride to the solution.
  4. Add 1.44 g of Sodium Phosphate Dibasic to the solution.
  5. Add 0.245 g of Potassium Phosphate Monobasic to the solution.
  6. Adjust solution to desired pH (typically pH ≈ 7.4).
  7. Add distilled water until the volume is 1 L.

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Physiological Buffer
pH Buffering
Sample Preparation
Cell/Culture/Growth Media
Gel Electrophoresis


This online tool may be cited as follows


"Quest Calculate™ PBS (Phosphate Buffered Saline) (1X, pH 7.4) Preparation and Recipe." AAT Bioquest, Inc.9 Jun2023https://www.aatbio.com/resources/buffer-preparations-and-recipes/pbs-phosphate-buffered-saline.


AAT Bioquest, Inc. (2023June 9). Quest Calculate™ PBS (Phosphate Buffered Saline) (1X, pH 7.4) Preparation and Recipe. AAT Bioquest. https://www.aatbio.com/resources/buffer-preparations-and-recipes/pbs-phosphate-buffered-saline.

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