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Glycine-Sodium Hydroxide Buffer (0.08 M, pH 10) Preparation and Recipe
A common buffer with good buffering capacity, often used in cellular biology. This buffer will slightly inhibit alkaline phosphatase enzymatic activity due to the configuration of the glycine amino group at higher pH levels. Due to this inhibition, it is recommended to use horseradish peroxidase (HRP) conjugates with this buffer.
To prepare
L of Glycine-Sodium Hydroxide Buffer (0.08 M, pH 10):
Change the value in the textbox above to scale the recipe volume
Table 1. Required components
ComponentAmountConcentration
Glycine (mw: 75.07 g / mol)##
Sodium Hydroxide (mw: 40 g / mol)##
  • Prepare 800 mL of distilled water in a suitable container.
  • Add # of Glycine to the solution
  • Add # of Sodium Hydroxide to the solution
  • Adjust solution to final desired pH using HCl or NaOH
  • Add distilled water until the volume is 1 L.
References
This online resource may be cited as follows
MLA
"Quest Calculate™ Glycine-Sodium Hydroxide Buffer (0.08 M, pH 10) Preparation and Recipe." AAT Bioquest, Inc., 13 Oct. 2025, https://www.aatbio.com/resources/buffer-preparations-and-recipes/glycine-sodium-hydroxide-buffer-ph-10.
APA
AAT Bioquest, Inc. (2025, October 13). Quest Calculate™ Glycine-Sodium Hydroxide Buffer (0.08 M, pH 10) Preparation and Recipe. AAT Bioquest. https://www.aatbio.com/resources/buffer-preparations-and-recipes/glycine-sodium-hydroxide-buffer-ph-10.
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