Glycine-Sodium Hydroxide Buffer (0.08 M, pH 10) Preparation and Recipe

A common buffer with good buffering capacity, often used in cellular biology. This buffer will slightly inhibit alkaline phosphatase enzymatic activity due to the configuration of the glycine amino group at higher pH levels. Due to this inhibition, it is recommended to use horseradish peroxidase (HRP) conjugates with this buffer.

To prepare L of Glycine-Sodium Hydroxide Buffer (0.08 M, pH 10):
Change the value in the textbox above to scale the recipe volume

Table 1. Required components

Component	Amount	Concentration
Glycine (mw: 75.07 g/mol)	6.01 g	0.08 M
Sodium Hydroxide (mw: 40.00 g/mol)	2.05 g	0.05125 M

Prepare 800 mL of distilled water in a suitable container.
Add 6.01 g of Glycine to the solution.
Add 2.05 g of Sodium Hydroxide to the solution.
Adjust solution to final desired pH using HCl or NaOH
Add distilled water until the volume is 1 L.

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Physiological Buffer

pH Buffering

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EBSS (magnesium, calcium, phenol red) (pH 7.0)
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Glycine-HCl Buffer (0.1 M, pH 3.0)
Glycine-Sodium Hydroxide Buffer (0.08 M, pH 10)
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References

This online tool may be cited as follows

MLA	"Quest Calculate™ Glycine-Sodium Hydroxide Buffer (0.08 M, pH 10) Preparation and Recipe." AAT Bioquest, Inc., 9 Dec. 2023, https://www.aatbio.com/resources/buffer-preparations-and-recipes/glycine-sodium-hydroxide-buffer-ph-10.
APA	AAT Bioquest, Inc. (2023, December 9). Quest Calculate™ Glycine-Sodium Hydroxide Buffer (0.08 M, pH 10) Preparation and Recipe. AAT Bioquest. https://www.aatbio.com/resources/buffer-preparations-and-recipes/glycine-sodium-hydroxide-buffer-ph-10.
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Glycine-Sodium Hydroxide Buffer (0.08 M, pH 10) Preparation and Recipe

References

This online tool may be cited as follows

MLA

APA