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Glycine-Sodium Hydroxide Buffer (0.08 M, pH 10) Preparation and Recipe

A common buffer with good buffering capacity, often used in cellular biology. This buffer will slightly inhibit alkaline phosphatase enzymatic activity due to the configuration of the glycine amino group at higher pH levels. Due to this inhibition, it is recommended to use horseradish peroxidase (HRP) conjugates with this buffer.

To prepare  L of Glycine-Sodium Hydroxide Buffer (0.08 M, pH 10):
Change the value in the textbox above to scale the recipe volume

Table 1. Required components
Glycine (mw: 75.07 g/mol)6.01 g0.08 M
Sodium Hydroxide (mw: 40.00 g/mol)2.05 g0.05125 M
  1. Prepare 800 mL of distilled water in a suitable container.
  2. Add 6.01 g of Glycine to the solution.
  3. Add 2.05 g of Sodium Hydroxide to the solution.
  4. Adjust solution to final desired pH using HCl or NaOH
  5. Add distilled water until the volume is 1 L.

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Physiological Buffer
pH Buffering
Sample Preparation
Cell/Culture/Growth Media
Gel Electrophoresis


This online tool may be cited as follows


"Quest Calculate™ Glycine-Sodium Hydroxide Buffer (0.08 M, pH 10) Preparation and Recipe." AAT Bioquest, Inc.26 May2024https://www.aatbio.com/resources/buffer-preparations-and-recipes/glycine-sodium-hydroxide-buffer-ph-10.


AAT Bioquest, Inc. (2024May 26). Quest Calculate™ Glycine-Sodium Hydroxide Buffer (0.08 M, pH 10) Preparation and Recipe. AAT Bioquest. https://www.aatbio.com/resources/buffer-preparations-and-recipes/glycine-sodium-hydroxide-buffer-ph-10.