Electrophoretic Transfer Buffer (20x) Preparation and Recipe

Electrophoretic Transfer Buffers are used in SDS-PAGE to transfer proteins from a gel to a nitrocellulose membrane.

To prepare

L of Electrophoretic Transfer Buffer (20x):

Change the value in the textbox above to scale the recipe volume

Table 1. Required components

Component	Amount	Concentration
Trisodium citrate (mw: 258.07 g / mol)	#	#
Sodium phosphate, dibasic (Na2HPO4) (mw: 141.96 g / mol)	#	#

Prepare 800 mL of distilled water in a suitable container.
Add # of Trisodium citrate to the solution
Add # of Sodium phosphate, dibasic (Na2HPO4) to the solution
Add distilled water until the volume is 1 L.
Adjust the pH to 8.3, autoclave, and store for up to 1-2 yr at room temperature.

References

This online resource may be cited as follows

MLA	"Quest Calculate™ Electrophoretic Transfer Buffer (20x) Preparation and Recipe." AAT Bioquest, Inc., 14 Oct. 2025, https://www.aatbio.com/resources/buffer-preparations-and-recipes/electrophoretic-transfer-buffer-20x.
APA	AAT Bioquest, Inc. (2025, October 14). Quest Calculate™ Electrophoretic Transfer Buffer (20x) Preparation and Recipe. AAT Bioquest. https://www.aatbio.com/resources/buffer-preparations-and-recipes/electrophoretic-transfer-buffer-20x.
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