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Electrophoretic Transfer Buffer (20x) Preparation and Recipe
Electrophoretic Transfer Buffers are used in SDS-PAGE to transfer proteins from a gel to a nitrocellulose membrane.
To prepare
L of Electrophoretic Transfer Buffer (20x):
Change the value in the textbox above to scale the recipe volume
Table 1. Required components
ComponentAmountConcentration
Trisodium citrate (mw: 258.07 g / mol)##
Sodium phosphate, dibasic (Na2HPO4) (mw: 141.96 g / mol)##
  • Prepare 800 mL of distilled water in a suitable container.
  • Add # of Trisodium citrate to the solution
  • Add # of Sodium phosphate, dibasic (Na2HPO4) to the solution
  • Add distilled water until the volume is 1 L.
  • Adjust the pH to 8.3, autoclave, and store for up to 1-2 yr at room temperature.


References
This online resource may be cited as follows
MLA
"Quest CalculateElectrophoretic Transfer Buffer (20x) Preparation and Recipe." AAT Bioquest, Inc.4 Oct2025https://www.aatbio.com/resources/buffer-preparations-and-recipes/electrophoretic-transfer-buffer-20x.
APA
AAT Bioquest, Inc. (2025October 4). Quest CalculateElectrophoretic Transfer Buffer (20x) Preparation and Recipe. AAT Bioquest. https://www.aatbio.com/resources/buffer-preparations-and-recipes/electrophoretic-transfer-buffer-20x.
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