AAT Bioquest

Electrophoretic Transfer Buffer (20x) Preparation and Recipe

Electrophoretic Transfer Buffers are used in SDS-PAGE to transfer proteins from a gel to a nitrocellulose membrane.

To prepare  L of Electrophoretic Transfer Buffer (20x):
Change the value in the textbox above to scale the recipe volume

Table 1. Required components
Trisodium citrate (mw: 258.07 g/mol)35.29 g0.12 M
Sodium phosphate, dibasic (Na2HPO4) (mw: 141.96 g/mol)22.71 g0.16 M
  1. Prepare 800 mL of distilled water in a suitable container.
  2. Add 35.29 g of Trisodium citrate to the solution.
  3. Add 22.71 g of Sodium phosphate, dibasic (Na2HPO4) to the solution.
  4. Add distilled water until the volume is 1 L.
  5. Adjust the pH to 8.3, autoclave, and store for up to 1-2 yr at room temperature.

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Physiological Buffer
pH Buffering
Sample Preparation
Cell/Culture/Growth Media
Gel Electrophoresis


This online tool may be cited as follows


"Quest Calculate™ Electrophoretic Transfer Buffer (20x) Preparation and Recipe." AAT Bioquest, Inc.16 Jun2024https://www.aatbio.com/resources/buffer-preparations-and-recipes/electrophoretic-transfer-buffer-20x.


AAT Bioquest, Inc. (2024June 16). Quest Calculate™ Electrophoretic Transfer Buffer (20x) Preparation and Recipe. AAT Bioquest. https://www.aatbio.com/resources/buffer-preparations-and-recipes/electrophoretic-transfer-buffer-20x.