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Tris-Glycine Transfer Buffer (20x) Preparation and Recipe

Tris-Glycine Transfer Buffer is used as a transfer buffer in Western blotting.

To prepare  L of Tris-Glycine Transfer Buffer (20x):
Change the value in the textbox above to scale the recipe volume

Table 1. Required components
Tris base (mw: 121.14 g/mol)24.2 g0.2 M
Glycine (mw: 75.07 g/mol)150.1 g2 M
  1. Prepare 800 mL of distilled water in a suitable container.
  2. Add 24.2 g of Tris base to the solution.
  3. Add 150.1 g of Glycine to the solution.
  4. Add distilled water until the volume is 1 L.
  5. pH adjustment is not necessary (it will be ~8.8). Store at room temperature.

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Physiological Buffer
pH Buffering
Sample Preparation
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This online tool may be cited as follows


"Quest Calculate™ Tris-Glycine Transfer Buffer (20x) Preparation and Recipe." AAT Bioquest, Inc.12 Jun2024https://www.aatbio.com/resources/buffer-preparations-and-recipes/tris-glycine-transfer-buffer-20x.


AAT Bioquest, Inc. (2024June 12). Quest Calculate™ Tris-Glycine Transfer Buffer (20x) Preparation and Recipe. AAT Bioquest. https://www.aatbio.com/resources/buffer-preparations-and-recipes/tris-glycine-transfer-buffer-20x.