Acrylamide Stock Solution Preparation and Recipe

Acrylamide is used along with bisacrylamide to form gels used in SDS-PAGE. Acrylamide forms polyacrylamide polymers, while bisacrylamide crosslinks between polyacrylamide chains.

To prepare

L of Acrylamide Stock Solution:

Change the value in the textbox above to scale the recipe volume

Table 1. Required components

Component	Amount	Concentration
acrylamide (mw: 71.08 g / mol)	#	#
bisacrylamide (mw: 154.17 g / mol)	#	#

Prepare 800 mL of distilled water in a suitable container.
Add # of acrylamide to the solution
Add # of bisacrylamide to the solution
Add distilled water until the volume is 1 L.
Filter the stock solution using Whatman filter paper and store at 4 °C. Prepare fresh acrylamide stock solution every few weeks.

References

This online resource may be cited as follows

MLA	"Quest Calculate™ Acrylamide Stock Solution Preparation and Recipe." AAT Bioquest, Inc., 22 Oct. 2025, https://www.aatbio.com/resources/buffer-preparations-and-recipes/acrylamide-stock-40-t-2-67-c.
APA	AAT Bioquest, Inc. (2025, October 22). Quest Calculate™ Acrylamide Stock Solution Preparation and Recipe. AAT Bioquest. https://www.aatbio.com/resources/buffer-preparations-and-recipes/acrylamide-stock-40-t-2-67-c.
BibTeX	EndNote	RefMan