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Acrylamide Stock Solution Preparation and Recipe

Acrylamide is used along with bisacrylamide to form gels used in SDS-PAGE. Acrylamide forms polyacrylamide polymers, while bisacrylamide crosslinks between polyacrylamide chains.

To prepare  L of Acrylamide Stock Solution:
Change the value in the textbox above to scale the recipe volume

Table 1. Required components
acrylamide (mw: 71.08 g/mol)389.6 g5.481 M
bisacrylamide (mw: 154.17 g/mol)10.4 g0.06745 M
  1. Prepare 800 mL of distilled water in a suitable container.
  2. Add 389.6 g of acrylamide to the solution.
  3. Add 10.4 g of bisacrylamide to the solution.
  4. Add distilled water until the volume is 1 L.
  5. Filter the stock solution using Whatman filter paper and store at 4°C. Prepare fresh acrylamide stock solution every few weeks. T denotes the total percentage concentration of the monomers (acrylamide plus N,N'-methylenebisacrylamide) in grams per 100 mL. C denotes the percentage (by weight) of the cross-linker N,N'-methylenebisacrylamide relative to the total amount of monomers.

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Physiological Buffer
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Sample Preparation
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This online tool may be cited as follows


"Quest Calculate™ Acrylamide Stock Solution Preparation and Recipe." AAT Bioquest, Inc.3 Feb2023


AAT Bioquest, Inc. (2023February 3). Quest Calculate™ Acrylamide Stock Solution Preparation and Recipe. AAT Bioquest.