AAT Bioquest

Acetate Buffer (pH 3.6 to 5.6) Preparation and Recipe

Sodium acetate buffers are used for purification and precipitation of nucleic acids, as well as for protein crystallization and staining gels used in protein electrophoresis. It is very popular in hematology, since there is some evidence that acetate-buffered infusions show improved stability. Acetate buffers are inexpensive and simple to prepare, and can be stored at room temperature.

To prepare  L of Acetate Buffer (pH 3.6 to 5.6):
Change the value in the textbox above to scale the recipe volume

Current molarity:
0.1 M
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Current pH:
Adjust the pH of the solution by using the slider below

Table 1. Required components
Sodium Acetate (mw: 82.03 g/mol)7.721 g0.09413 M
Acetic Acid (mw: 60.05 g/mol)352.5 mg0.005871 M
  1. Prepare 800 mL of distilled water in a suitable container.
  2. Add 7.721 g of Sodium Acetate to the solution.
  3. Add 352.5 mg of Acetic Acid to the solution.
  4. Adjust solution to final desired pH using HCl or NaOH
  5. Add distilled water until the volume is 1 L.

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Physiological Buffer
pH Buffering
Sample Preparation
Cell/Culture/Growth Media
Gel Electrophoresis


This online tool may be cited as follows


"Quest Calculate™ Acetate Buffer (pH 3.6 to 5.6) Preparation and Recipe." AAT Bioquest, Inc.10 Jun2023https://www.aatbio.com/resources/buffer-preparations-and-recipes/acetate-buffer-ph-3-6-to-5-6.


AAT Bioquest, Inc. (2023June 10). Quest Calculate™ Acetate Buffer (pH 3.6 to 5.6) Preparation and Recipe. AAT Bioquest. https://www.aatbio.com/resources/buffer-preparations-and-recipes/acetate-buffer-ph-3-6-to-5-6.

This online tool has been cited in 2 publications, including

Precursor and PGG Concentration Affects the Tropoelastin Coacervation Peak Absorbance
Journal: (2022)
Biotranformation Of Environmental Toxicants By Different Enzymes
Journal: (2022)