Acetate Buffer (pH 3.6 to 5.6) Preparation and Recipe
Sodium acetate buffers are used for purification and precipitation of nucleic acids, as well as for protein crystallization and staining gels used in protein electrophoresis. It is very popular in hematology, since there is some evidence that acetate-buffered infusions show improved stability. Acetate buffers are inexpensive and simple to prepare, and can be stored at room temperature.
To prepare L of Acetate Buffer (pH 3.6 to 5.6):
Change the value in the textbox above to scale the recipe volume
Change the value in the textbox above to scale the recipe volume
Current molarity: 0.1 M | Adjust the molarity of the solution by using the slider below |
Current pH: 5.6 | Adjust the pH of the solution by using the slider below |
Table 1. Required components
Component | Amount | Concentration |
Sodium Acetate (mw: 82.03 g/mol) | 7.721 g | 0.09413 M |
Acetic Acid (mw: 60.05 g/mol) | 352.5 mg | 0.005871 M |
- Prepare 800 mL of distilled water in a suitable container.
- Add 7.721 g of Sodium Acetate to the solution.
- Add 352.5 mg of Acetic Acid to the solution.
- Adjust solution to final desired pH using HCl or NaOH
- Add distilled water until the volume is 1 L.
Physiological Buffer
pH Buffering
Sample Preparation
BioAssays
Misc
Cell/Culture/Growth Media
Gel Electrophoresis
References
This online tool may be cited as follows
MLA | "Quest Calculate™ Acetate Buffer (pH 3.6 to 5.6) Preparation and Recipe." AAT Bioquest, Inc., 11 Dec. 2024, https://www.aatbio.com/resources/buffer-preparations-and-recipes/acetate-buffer-ph-3-6-to-5-6. | |
APA | AAT Bioquest, Inc. (2024, December 11). Quest Calculate™ Acetate Buffer (pH 3.6 to 5.6) Preparation and Recipe. AAT Bioquest. https://www.aatbio.com/resources/buffer-preparations-and-recipes/acetate-buffer-ph-3-6-to-5-6. | |
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