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Potassium Phosphate (pH 5.8 to 8.0) Preparation and Recipe

Potassium phosphate buffers, sometimes called Gomori buffers, consist of a mixture of monobasic dihydrogen phosphate and dibasic monohydrogen phosphate. These buffers have excellent buffering capacity and are highly soluble in water. However, potassium phosphate buffer reacts with some metal ions including calcium and magnesium, and inhibits enzymatic reactions. Additionally, all phosphates precipitate in ethanol, which can be deleterious to some nucleic acid preparations that typically use ethanol solutions. Potassium phosphate buffer is used in research fields ranging from molecular biology to botany, due to its adaptability and affordability.

To prepare  L of Potassium Phosphate (pH 5.8 to 8.0):
Change the value in the textbox above to scale the recipe volume

Current molarity:
0.1 M
Adjust the molarity of the solution by using the slider below
Current pH:
8
Adjust the pH of the solution by using the slider below

Table 1. Required components
ComponentAmountConcentration
Potassium phosphate dibasic (mw: 174.18 g/mol)16.282 g0.09348 M
Potassium Phosphate Monobasic (mw: 136.09 g/mol)887.8 mg0.006523 M
  1. Prepare 800 mL of dH2O in a suitable container.
  2. Add 16.282 g of Potassium phosphate dibasic to the solution.
  3. Add 887.8 mg of Potassium Phosphate Monobasic to the solution.
  4. Add dH2O until the volume is 1 L.

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Physiological Buffer
pH Buffering
Sample Preparation
BioAssays
Misc
Cell/Culture/Growth Media
Gel Electrophoresis


References

This online tool may be cited as follows

MLA

"Quest Calculate™ Potassium Phosphate (pH 5.8 to 8.0) Preparation and Recipe." AAT Bioquest, Inc.15 Aug2022https://www.aatbio.com/resources/buffer-preparations-and-recipes/potassium-phosphate-ph-5-8-to-8-0.

APA

AAT Bioquest, Inc. (2022August 15). Quest Calculate™ Potassium Phosphate (pH 5.8 to 8.0) Preparation and Recipe. AAT Bioquest. https://www.aatbio.com/resources/buffer-preparations-and-recipes/potassium-phosphate-ph-5-8-to-8-0.