AAT Bioquest

Potassium Phosphate (pH 5.8 to 8.0) Preparation and Recipe

Potassium phosphate buffers, sometimes called Gomori buffers, consist of a mixture of monobasic dihydrogen phosphate and dibasic monohydrogen phosphate. These buffers have excellent buffering capacity and are highly soluble in water. However, potassium phosphate buffer reacts with some metal ions including calcium and magnesium, and inhibits enzymatic reactions. Additionally, all phosphates precipitate in ethanol, which can be deleterious to some nucleic acid preparations that typically use ethanol solutions. Potassium phosphate buffer is used in research fields ranging from molecular biology to botany, due to its adaptability and affordability.

To prepare  L of Potassium Phosphate (pH 5.8 to 8.0):
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Current molarity:
0.1 M
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Current pH:
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Table 1. Required components
Potassium phosphate dibasic (mw: 174.18 g/mol)16.282 g0.09348 M
Potassium Phosphate Monobasic (mw: 136.09 g/mol)887.8 mg0.006523 M
  1. Prepare 800 mL of dH2O in a suitable container.
  2. Add 16.282 g of Potassium phosphate dibasic to the solution.
  3. Add 887.8 mg of Potassium Phosphate Monobasic to the solution.
  4. Add dH2O until the volume is 1 L.

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Physiological Buffer
pH Buffering
Sample Preparation
Cell/Culture/Growth Media
Gel Electrophoresis


This online tool may be cited as follows


"Quest Calculate™ Potassium Phosphate (pH 5.8 to 8.0) Preparation and Recipe." AAT Bioquest, Inc.20 Apr2024https://www.aatbio.com/resources/buffer-preparations-and-recipes/potassium-phosphate-ph-5-8-to-8-0.


AAT Bioquest, Inc. (2024April 20). Quest Calculate™ Potassium Phosphate (pH 5.8 to 8.0) Preparation and Recipe. AAT Bioquest. https://www.aatbio.com/resources/buffer-preparations-and-recipes/potassium-phosphate-ph-5-8-to-8-0.

This online tool has been cited in 1 publications, including

Detailed characterization of the cell wall structure and composition of nordic green microalgae
Journal: Journal of agricultural and food chemistry (2022): 9711--9721