TBS (1 M, pH 7.4) Preparation and Recipe

Tris-Buffered Saline (TBS) is a popular isotonic buffer used for multiple applications, including as a washing buffer in immunoassays of all kinds. It is one of the default buffers for antibody preparation. For other antibody preparation materials, click here.

To prepare

L of TBS (1 M, pH 7.4):

Change the value in the textbox above to scale the recipe volume

Table 1. Required components

Component	Amount	Concentration
Sodium chloride (mw: 58.44 g / mol)	#	#
Potassium Chloride (mw: 74.55 g / mol)	#	#
Tris base (mw: 121.14 g / mol)	#	#

Prepare 800 mL of dH2O in a suitable container.
Add # of Sodium chloride to the solution
Add # of Potassium Chloride to the solution
Add # of Tris base to the solution
Adjust solution to desired pH with HCl (typically 7.4).
Add dH2O until the volume is 1 L.

References

This online resource may be cited as follows

MLA	"Quest Calculate™ TBS (1 M, pH 7.4) Preparation and Recipe." AAT Bioquest, Inc., 5 Nov. 2025, https://www.aatbio.com/resources/buffer-preparations-and-recipes/tbs-ph-7-4.
APA	AAT Bioquest, Inc. (2025, November 5). Quest Calculate™ TBS (1 M, pH 7.4) Preparation and Recipe. AAT Bioquest. https://www.aatbio.com/resources/buffer-preparations-and-recipes/tbs-ph-7-4.
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