AAT Bioquest

TBS (1 M, pH 7.4) Preparation and Recipe

Tris-Buffered Saline (TBS) is a popular isotonic buffer used for multiple applications, including as a washing buffer in immunoassays of all kinds. It is one of the default buffers for antibody preparation. For other antibody preparation materials, click here.

To prepare  L of TBS (1 M, pH 7.4):
Change the value in the textbox above to scale the recipe volume

Table 1. Required components
Sodium chloride (mw: 58.44 g/mol)8 g0.137 M
Potassium Chloride (mw: 74.55 g/mol)0.2 g0.00268 M
Tris base (mw: 121.14 g/mol)3 g0.0248 M
  1. Prepare 800 mL of dH2O in a suitable container.
  2. Add 8 g of Sodium chloride to the solution.
  3. Add 0.2 g of Potassium Chloride to the solution.
  4. Add 3 g of Tris base to the solution.
  5. Adjust solution to desired pH with HCl (typically 7.4).
  6. Add dH2O until the volume is 1 L.

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Physiological Buffer
pH Buffering
Sample Preparation
Cell/Culture/Growth Media
Gel Electrophoresis


This online tool may be cited as follows


"Quest Calculate™ TBS (1 M, pH 7.4) Preparation and Recipe." AAT Bioquest, Inc.25 May2024https://www.aatbio.com/resources/buffer-preparations-and-recipes/tbs-ph-7-4.


AAT Bioquest, Inc. (2024May 25). Quest Calculate™ TBS (1 M, pH 7.4) Preparation and Recipe. AAT Bioquest. https://www.aatbio.com/resources/buffer-preparations-and-recipes/tbs-ph-7-4.