AAT Bioquest

M9 Minimal Salts Preparation and Recipe

To prepare  L of M9 Minimal Salts:
Change the value in the textbox above to scale the recipe volume

Table 1. Required components
Sodium Phosphate Dibasic Heptahydrate (mw: 268.07 g/mol)64 g0.2387 M
Potassium Phosphate Monobasic (mw: 136.09 g/mol)15 g0.1102 M
Sodium chloride (mw: 58.44 g/mol)2.5 g0.04277 M
Ammonium chloride (mw: 53.49 g/mol)5 g0.09347 M
  1. Prepare 800 mL of distilled water in a suitable container.
  2. Add 64 g of Sodium Phosphate Dibasic Heptahydrate to the solution.
  3. Add 15 g of Potassium Phosphate Monobasic to the solution.
  4. Add 2.5 g of Sodium chloride to the solution.
  5. Add 5 g of Ammonium chloride to the solution.
  6. Add distilled water until the volume is 1 L.
  7. Divide the salt solution into 200-ml aliquots and sterilize by autoclaving for 15 minutes at 15 psi (1.05 kg/cm 2) on liquid cycle.

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This online tool may be cited as follows


"Quest Calculate™ M9 Minimal Salts Preparation and Recipe." AAT Bioquest, Inc.24 May2024https://www.aatbio.com/resources/buffer-preparations-and-recipes/m9-salts.


AAT Bioquest, Inc. (2024May 24). Quest Calculate™ M9 Minimal Salts Preparation and Recipe. AAT Bioquest. https://www.aatbio.com/resources/buffer-preparations-and-recipes/m9-salts.

This online tool has been cited in 1 publications, including

Temperature-and Nutrients-Induced Phenotypic Changes of Antarctic Green Snow Bacteria Probed by High-Throughput FTIR Spectroscopy
Journal: Biology (2022): 890