M9 Minimal Salts Preparation and Recipe

To prepare

L of M9 Minimal Salts:

Change the value in the textbox above to scale the recipe volume

Table 1. Required components

Component	Amount	Concentration
Sodium Phosphate Dibasic Heptahydrate (mw: 268.07 g / mol)	#	#
Potassium Phosphate Monobasic (mw: 136.09 g / mol)	#	#
Sodium chloride (mw: 58.44 g / mol)	#	#
Ammonium chloride (mw: 53.49 g / mol)	#	#

Prepare 800 mL of distilled water in a suitable container.
Add # of Sodium Phosphate Dibasic Heptahydrate to the solution
Add # of Potassium Phosphate Monobasic to the solution
Add # of Sodium chloride to the solution
Add # of Ammonium chloride to the solution
Add distilled water until the volume is 1 L.
Divide the salt solution into 200-ml aliquots and sterilize by autoclaving for 15 minutes at 15 psi (1.05 kg/cm 2) on liquid cycle.

References

This online resource may be cited as follows

MLA	"Quest Calculate™ M9 Minimal Salts Preparation and Recipe." AAT Bioquest, Inc., 28 Oct. 2025, https://www.aatbio.com/resources/buffer-preparations-and-recipes/m9-salts.
APA	AAT Bioquest, Inc. (2025, October 28). Quest Calculate™ M9 Minimal Salts Preparation and Recipe. AAT Bioquest. https://www.aatbio.com/resources/buffer-preparations-and-recipes/m9-salts.
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This online resource has been cited in 1 publications, including

Authors:

Smirnova, Margarita and Tafintseva, Valeria and Kohler, Achim and Miamin, Uladzislau and Shapaval, Volha

Journal:

Biology (2022): 890