Tyrode's Solution, Acidic Preparation and Recipe

To prepare

L of Tyrode's Solution, Acidic:

Change the value in the textbox above to scale the recipe volume

Table 1. Required components

Component	Amount	Concentration
Sodium chloride (mw: 58.44 g / mol)	#	#
Potassium Chloride (mw: 74.55 g / mol)	#	#
Calcium chloride dihydrate (mw: 147.01 g / mol)	#	#
Magnesium Chloride Hexahydrate (mw: 203.3 g / mol)	#	#
Glucose (mw: 180.16 g / mol)	#	#
Polyvinylpyrrolidone (PVP) (mw: 40000 g / mol)	#	#

Prepare 800 mL of distilled water in a suitable container.
Add # of Sodium chloride to the solution
Add # of Potassium Chloride to the solution
Add # of Calcium chloride dihydrate to the solution
Add # of Magnesium Chloride Hexahydrate to the solution
Add # of Glucose to the solution
Add # of Polyvinylpyrrolidone (PVP) to the solution
Add distilled water until the volume is 1 L.
Prepare at room temperature and adjust to pH 2.5 with Analar HCl (BDH). The PVP is added to increase viscosity and reduce embryo stickiness. Filter-sterilize and store in aliquots at -20?C. Acidic Tyrode?s is also available from Sigma and Specialty Media.

References

This online resource may be cited as follows

MLA	"Quest Calculate™ Tyrode's Solution, Acidic Preparation and Recipe." AAT Bioquest, Inc., 7 Nov. 2025, https://www.aatbio.com/resources/buffer-preparations-and-recipes/tyrode-s-solution-acidic.
APA	AAT Bioquest, Inc. (2025, November 7). Quest Calculate™ Tyrode's Solution, Acidic Preparation and Recipe. AAT Bioquest. https://www.aatbio.com/resources/buffer-preparations-and-recipes/tyrode-s-solution-acidic.
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This online resource has been cited in 1 publications, including

Authors:

Lawal, Babatunde AS and Odoala, Finian K

Journal:

Tropical Journal of Pharmaceutical Research (2023): 2327--2332