Tris Buffer (1 M, pH 7.2) Preparation and Recipe

Tricine is derived from the amino acids tris and glycine. It is a dipolar ion (Zwitterionic) and hydroxyl radical scavenger, and is used extensively for SDS-PAGE applications for small proteins. Tricine buffer is also commonly used for electrophoresis procedures.

To prepare

L of Tris Buffer (1 M, pH 7.2):

Change the value in the textbox above to scale the recipe volume

Table 1. Required components

Component	Amount	Concentration
Tris base (mw: 121.14 g / mol)	#	#

Prepare 800 mL of distilled water in a suitable container.
Add # of Tris base to the solution
Adjust solution to desired pH using HCl (typically pH ≈ 7.0).
Add distilled water until the volume is 1 L.

References

This online resource may be cited as follows

MLA	"Quest Calculate™ Tris Buffer (1 M, pH 7.2) Preparation and Recipe." AAT Bioquest, Inc., 21 Oct. 2025, https://www.aatbio.com/resources/buffer-preparations-and-recipes/tris-buffer.
APA	AAT Bioquest, Inc. (2025, October 21). Quest Calculate™ Tris Buffer (1 M, pH 7.2) Preparation and Recipe. AAT Bioquest. https://www.aatbio.com/resources/buffer-preparations-and-recipes/tris-buffer.
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