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Tris Buffer (1 M, pH 7.2) Preparation and Recipe
Tricine is derived from the amino acids tris and glycine. It is a dipolar ion (Zwitterionic) and hydroxyl radical scavenger, and is used extensively for SDS-PAGE applications for small proteins. Tricine buffer is also commonly used for electrophoresis procedures.
To prepare
L of Tris Buffer (1 M, pH 7.2):
Change the value in the textbox above to scale the recipe volume
Table 1. Required components
ComponentAmountConcentration
Tris base (mw: 121.14 g / mol)##
  • Prepare 800 mL of distilled water in a suitable container.
  • Add # of Tris base to the solution
  • Adjust solution to desired pH using HCl (typically pH ≈ 7.0).
  • Add distilled water until the volume is 1 L.


References
This online resource may be cited as follows
MLA
"Quest CalculateTris Buffer (1 M, pH 7.2) Preparation and Recipe." AAT Bioquest, Inc.8 Oct2025https://www.aatbio.com/resources/buffer-preparations-and-recipes/tris-buffer.
APA
AAT Bioquest, Inc. (2025October 8). Quest CalculateTris Buffer (1 M, pH 7.2) Preparation and Recipe. AAT Bioquest. https://www.aatbio.com/resources/buffer-preparations-and-recipes/tris-buffer.
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