AAT Bioquest

Tris Buffer (1 M, pH 7.2) Preparation and Recipe

Tricine is derived from the amino acids tris and glycine. It is a dipolar ion (Zwitterionic) and hydroxyl radical scavenger, and is used extensively for SDS-PAGE applications for small proteins. Tricine buffer is also commonly used for electrophoresis procedures.

To prepare  L of Tris Buffer (1 M, pH 7.2):
Change the value in the textbox above to scale the recipe volume

Table 1. Required components
Tris base (mw: 121.14 g/mol)121.14 g1 M
  1. Prepare 800 mL of distilled water in a suitable container.
  2. Add 121.14 g of Tris base to the solution.
  3. Adjust solution to desired pH using HCl (typically pH ≈ 7.0).
  4. Add distilled water until the volume is 1 L.

Physiological Buffer
pH Buffering
Sample Preparation
Cell/Culture/Growth Media
Gel Electrophoresis


This online tool may be cited as follows


"Quest Calculate™ Tris Buffer (1 M, pH 7.2) Preparation and Recipe." AAT Bioquest, Inc.13 Jul2024https://www.aatbio.com/resources/buffer-preparations-and-recipes/tris-buffer.


AAT Bioquest, Inc. (2024July 13). Quest Calculate™ Tris Buffer (1 M, pH 7.2) Preparation and Recipe. AAT Bioquest. https://www.aatbio.com/resources/buffer-preparations-and-recipes/tris-buffer.