Hydrochloric Acid-Potassium Chloride Buffer (0.1 M, pH 2.0) Preparation and Recipe
This common buffer is used in multiple biology research fields, often for enzymatic or histochemical usage. It should ideally be prepared shortly before use, as it does not store well.
To prepare L of Hydrochloric Acid-Potassium Chloride Buffer (0.1 M, pH 2.0):
Change the value in the textbox above to scale the recipe volume
Change the value in the textbox above to scale the recipe volume
Table 1. Required components
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Component | Amount | Concentration |
Potassium Chloride (mw: 74.55 g/mol) | 7.45 g | 0.1 M |
Hydrochloric acid (mw: 36.46 g/mol) | 0.772 g | 0.02 M |
- Prepare 800 mL of distilled water in a suitable container.
- Add 7.45 g of Potassium Chloride to the solution.
- Add 0.772 g of Hydrochloric acid to the solution.
- Adjust solution to final desired pH using HCl or NaOH
- Add distilled water until the volume is 1 L.
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Physiological Buffer
pH Buffering
Sample Preparation
BioAssays
Misc
Cell/Culture/Growth Media
Gel Electrophoresis
References
This online tool may be cited as follows
MLA | "Quest Calculate™ Hydrochloric Acid-Potassium Chloride Buffer (0.1 M, pH 2.0) Preparation and Recipe." AAT Bioquest, Inc., 6 Dec. 2023, https://www.aatbio.com/resources/buffer-preparations-and-recipes/hydrochloric-acid-potassium-chloride-buffer-ph-2. | |
APA | AAT Bioquest, Inc. (2023, December 6). Quest Calculate™ Hydrochloric Acid-Potassium Chloride Buffer (0.1 M, pH 2.0) Preparation and Recipe. AAT Bioquest. https://www.aatbio.com/resources/buffer-preparations-and-recipes/hydrochloric-acid-potassium-chloride-buffer-ph-2. | |
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