Actively helping customers, employees and the global community during the coronavirus SARS-CoV-2 outbreak.  Learn more >>

Hydrochloric Acid-Potassium Chloride Buffer (0.1 M, pH 2.0) Preparation and Recipe

This common buffer is used in multiple biology research fields, often for enzymatic or histochemical usage. It should ideally be prepared shortly before use, as it does not store well.

To prepare  L of Hydrochloric Acid-Potassium Chloride Buffer (0.1 M, pH 2.0):
Change the value in the textbox above to scale the recipe volume

Table 1. Required components
ComponentAmountConcentration
Potassium Chloride (mw: 74.55 g/mol)7.45 g0.1 M
Hydrochloric acid (mw: 36.46 g/mol)0.772 g0.02 M
  1. Prepare 800 mL of distilled water in a suitable container.
  2. Add 7.45 g of Potassium Chloride to the solution.
  3. Add 0.772 g of Hydrochloric acid to the solution.
  4. Adjust solution to final desired pH using HCl or NaOH
  5. Add distilled water until the volume is 1 L.

To make a purchase inquiry for this buffer, please provide your email address below:
Request quotation



Physiological Buffer
pH Buffering
Sample Preparation
BioAssays
Misc
Cell/Culture/Growth Media
Gel Electrophoresis


References

This online tool may be cited as follows

MLA

"Quest Calculate™ Hydrochloric Acid-Potassium Chloride Buffer (0.1 M, pH 2.0) Preparation and Recipe." AAT Bioquest, Inc.10 Aug2022https://www.aatbio.com/resources/buffer-preparations-and-recipes/hydrochloric-acid-potassium-chloride-buffer-ph-2.

APA

AAT Bioquest, Inc. (2022August 10). Quest Calculate™ Hydrochloric Acid-Potassium Chloride Buffer (0.1 M, pH 2.0) Preparation and Recipe. AAT Bioquest. https://www.aatbio.com/resources/buffer-preparations-and-recipes/hydrochloric-acid-potassium-chloride-buffer-ph-2.