AAT Bioquest

Hydrochloric Acid-Potassium Chloride Buffer (0.1 M, pH 2.0) Preparation and Recipe

This common buffer is used in multiple biology research fields, often for enzymatic or histochemical usage. It should ideally be prepared shortly before use, as it does not store well.

To prepare  L of Hydrochloric Acid-Potassium Chloride Buffer (0.1 M, pH 2.0):
Change the value in the textbox above to scale the recipe volume

Table 1. Required components
Potassium Chloride (mw: 74.55 g/mol)7.45 g0.1 M
Hydrochloric acid (mw: 36.46 g/mol)0.772 g0.02 M
  1. Prepare 800 mL of distilled water in a suitable container.
  2. Add 7.45 g of Potassium Chloride to the solution.
  3. Add 0.772 g of Hydrochloric acid to the solution.
  4. Adjust solution to final desired pH using HCl or NaOH
  5. Add distilled water until the volume is 1 L.

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Physiological Buffer
pH Buffering
Sample Preparation
Cell/Culture/Growth Media
Gel Electrophoresis


This online tool may be cited as follows


"Quest Calculate™ Hydrochloric Acid-Potassium Chloride Buffer (0.1 M, pH 2.0) Preparation and Recipe." AAT Bioquest, Inc.10 Jun2023https://www.aatbio.com/resources/buffer-preparations-and-recipes/hydrochloric-acid-potassium-chloride-buffer-ph-2.


AAT Bioquest, Inc. (2023June 10). Quest Calculate™ Hydrochloric Acid-Potassium Chloride Buffer (0.1 M, pH 2.0) Preparation and Recipe. AAT Bioquest. https://www.aatbio.com/resources/buffer-preparations-and-recipes/hydrochloric-acid-potassium-chloride-buffer-ph-2.