Hydrochloric Acid-Potassium Chloride Buffer (0.1 M, pH 2.0) Preparation and Recipe

This common buffer is used in multiple biology research fields, often for enzymatic or histochemical usage. It should ideally be prepared shortly before use, as it does not store well.

To prepare

L of Hydrochloric Acid-Potassium Chloride Buffer (0.1 M, pH 2.0):

Change the value in the textbox above to scale the recipe volume

Table 1. Required components

Component	Amount	Concentration
Potassium Chloride (mw: 74.55 g / mol)	#	#
Hydrochloric acid (mw: 36.46 g / mol)	#	#

Prepare 800 mL of distilled water in a suitable container.
Add # of Potassium Chloride to the solution
Add # of Hydrochloric acid to the solution
Adjust solution to final desired pH using HCl or NaOH
Add distilled water until the volume is 1 L.

References

This online resource may be cited as follows

MLA	"Quest Calculate™ Hydrochloric Acid-Potassium Chloride Buffer (0.1 M, pH 2.0) Preparation and Recipe." AAT Bioquest, Inc., 6 Nov. 2025, https://www.aatbio.com/resources/buffer-preparations-and-recipes/hydrochloric-acid-potassium-chloride-buffer-ph-2.
APA	AAT Bioquest, Inc. (2025, November 6). Quest Calculate™ Hydrochloric Acid-Potassium Chloride Buffer (0.1 M, pH 2.0) Preparation and Recipe. AAT Bioquest. https://www.aatbio.com/resources/buffer-preparations-and-recipes/hydrochloric-acid-potassium-chloride-buffer-ph-2.
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