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5-Fluorocytosine (5-FC) Solution Preparation and Recipe

5-Fluorocytosine (5-FC) is a commonly used antifungal that inhibits DNA and RNA synthesis.

To prepare  L of 5-Fluorocytosine (5-FC) Solution:
Change the value in the textbox above to scale the recipe volume

Table 1. Required components
ComponentAmountConcentration
5-FC (mw: 129.09 g/mol)10 g0.07746 M
  1. Prepare 800 mL of distilled water in a suitable container.
  2. Add 10 g of 5-FC to the solution.
  3. Add distilled water until the volume is 1 L.
  4. Vortex. Incubate for 2 h at 37°C, manually mixing the solution every 15 min until the 5-FC dissolves. Filter the solution and use it immediately. Alternatively, aliquot into sterile tubes and store at -20°C.

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Physiological Buffer
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Sample Preparation
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Misc
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Gel Electrophoresis


References

This online tool may be cited as follows

MLA

"Quest Calculate™ 5-Fluorocytosine (5-FC) Solution Preparation and Recipe." AAT Bioquest, Inc.1 Oct2022https://www.aatbio.com/resources/buffer-preparations-and-recipes/5-fluorocytosine-5-fc-10-mg-ml.

APA

AAT Bioquest, Inc. (2022October 1). Quest Calculate™ 5-Fluorocytosine (5-FC) Solution Preparation and Recipe. AAT Bioquest. https://www.aatbio.com/resources/buffer-preparations-and-recipes/5-fluorocytosine-5-fc-10-mg-ml.