5-Fluorocytosine (5-FC) Solution Preparation and Recipe

5-Fluorocytosine (5-FC) is a commonly used antifungal that inhibits DNA and RNA synthesis.

To prepare

L of 5-Fluorocytosine (5-FC) Solution:

Change the value in the textbox above to scale the recipe volume

Table 1. Required components

Component	Amount	Concentration
5-FC (mw: 129.09 g / mol)	#	#

Prepare 800 mL of distilled water in a suitable container.
Add # of 5-FC to the solution
Add distilled water until the volume is 1 L.
Vortex. Incubate for 2 h at 37°C, manually mixing the solution every 15 min until the 5-FC dissolves. Filter the solution and use it immediately. Alternatively, aliquot into sterile tubes and store at -20°C.

References

This online resource may be cited as follows

MLA	"Quest Calculate™ 5-Fluorocytosine (5-FC) Solution Preparation and Recipe." AAT Bioquest, Inc., 5 Dec. 2025, https://www.aatbio.com/resources/buffer-preparations-and-recipes/5-fluorocytosine-5-fc-10-mg-ml.
APA	AAT Bioquest, Inc. (2025, December 5). Quest Calculate™ 5-Fluorocytosine (5-FC) Solution Preparation and Recipe. AAT Bioquest. https://www.aatbio.com/resources/buffer-preparations-and-recipes/5-fluorocytosine-5-fc-10-mg-ml.
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