AAT Bioquest

5-Fluorocytosine (5-FC) Solution Preparation and Recipe

5-Fluorocytosine (5-FC) is a commonly used antifungal that inhibits DNA and RNA synthesis.

To prepare  L of 5-Fluorocytosine (5-FC) Solution:
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Table 1. Required components
5-FC (mw: 129.09 g/mol)10 g0.07746 M
  1. Prepare 800 mL of distilled water in a suitable container.
  2. Add 10 g of 5-FC to the solution.
  3. Add distilled water until the volume is 1 L.
  4. Vortex. Incubate for 2 h at 37°C, manually mixing the solution every 15 min until the 5-FC dissolves. Filter the solution and use it immediately. Alternatively, aliquot into sterile tubes and store at -20°C.

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This online tool may be cited as follows


"Quest Calculate™ 5-Fluorocytosine (5-FC) Solution Preparation and Recipe." AAT Bioquest, Inc.16 Jun2024https://www.aatbio.com/resources/buffer-preparations-and-recipes/5-fluorocytosine-5-fc-10-mg-ml.


AAT Bioquest, Inc. (2024June 16). Quest Calculate™ 5-Fluorocytosine (5-FC) Solution Preparation and Recipe. AAT Bioquest. https://www.aatbio.com/resources/buffer-preparations-and-recipes/5-fluorocytosine-5-fc-10-mg-ml.