AAT Bioquest

Bath Solution (Ca2+/Mg2+ Free) Preparation and Recipe

To prepare  L of Bath Solution (Ca2+/Mg2+ Free):
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Table 1. Required components
Potassium hydroxide (mw: 56.106 g/mol)7.854 g0.14 M
Hepes (mw: 238.30 g/mol)4.766 g0.02 M
Potassium Chloride (mw: 74.55 g/mol)0.1491 g0.002 M
EGTA (mw: 380.35 g/mol)1.902 g0.005 M
Methanesulfonic acid (mw: 96.11 g/mol)13.314 g0.14 M
  1. Prepare 800 mL of distilled water in a suitable container.
  2. Add 7.854 g of Potassium hydroxide to the solution.
  3. Add 4.766 g of Hepes to the solution.
  4. Add 0.1491 g of Potassium Chloride to the solution.
  5. Add 1.902 g of EGTA to the solution.
  6. Add 13.314 g of Methanesulfonic acid to the solution.
  7. Add distilled water until the volume is 1 L.
  8. Use methanesulfonic acid to adjust the pH to 7.1-7.2. Store for up to 3 years at room temperature (22°C-24°C).

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Physiological Buffer
pH Buffering
Sample Preparation
Cell/Culture/Growth Media
Gel Electrophoresis


This online tool may be cited as follows


"Quest Calculate™ Bath Solution (Ca2+/Mg2+ Free) Preparation and Recipe." AAT Bioquest, Inc.15 Apr2024https://www.aatbio.com/resources/buffer-preparations-and-recipes/bath-solution-ca2-mg2-free.


AAT Bioquest, Inc. (2024April 15). Quest Calculate™ Bath Solution (Ca2+/Mg2+ Free) Preparation and Recipe. AAT Bioquest. https://www.aatbio.com/resources/buffer-preparations-and-recipes/bath-solution-ca2-mg2-free.