Bath Solution (Ca2+/Mg2+ Free) Preparation and Recipe
To prepare L of Bath Solution (Ca2+/Mg2+ Free):
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Table 1. Required components
Component | Amount | Concentration |
Potassium hydroxide (mw: 56.106 g/mol) | 7.854 g | 0.14 M |
Hepes (mw: 238.30 g/mol) | 4.766 g | 0.02 M |
Potassium Chloride (mw: 74.55 g/mol) | 0.1491 g | 0.002 M |
EGTA (mw: 380.35 g/mol) | 1.902 g | 0.005 M |
Methanesulfonic acid (mw: 96.11 g/mol) | 13.314 g | 0.14 M |
- Prepare 800 mL of distilled water in a suitable container.
- Add 7.854 g of Potassium hydroxide to the solution.
- Add 4.766 g of Hepes to the solution.
- Add 0.1491 g of Potassium Chloride to the solution.
- Add 1.902 g of EGTA to the solution.
- Add 13.314 g of Methanesulfonic acid to the solution.
- Add distilled water until the volume is 1 L.
- Use methanesulfonic acid to adjust the pH to 7.1-7.2. Store for up to 3 years at room temperature (22°C-24°C).
Physiological Buffer
pH Buffering
Sample Preparation
BioAssays
Misc
Cell/Culture/Growth Media
Gel Electrophoresis
References
This online tool may be cited as follows
MLA | "Quest Calculate™ Bath Solution (Ca2+/Mg2+ Free) Preparation and Recipe." AAT Bioquest, Inc., 13 Nov. 2024, https://www.aatbio.com/resources/buffer-preparations-and-recipes/bath-solution-ca2-mg2-free. | |
APA | AAT Bioquest, Inc. (2024, November 13). Quest Calculate™ Bath Solution (Ca2+/Mg2+ Free) Preparation and Recipe. AAT Bioquest. https://www.aatbio.com/resources/buffer-preparations-and-recipes/bath-solution-ca2-mg2-free. | |
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