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Bath Solution (Ca2+/Mg2+ Free) Preparation and Recipe
To prepare
L of Bath Solution (Ca2+/Mg2+ Free):
Change the value in the textbox above to scale the recipe volume
Table 1. Required components
ComponentAmountConcentration
Potassium hydroxide (mw: 56.106 g / mol)##
Hepes (mw: 238.3 g / mol)##
Potassium Chloride (mw: 74.55 g / mol)##
EGTA (mw: 380.35 g / mol)##
Methanesulfonic acid (mw: 96.11 g / mol)##
  • Prepare 800 mL of distilled water in a suitable container.
  • Add # of Potassium hydroxide to the solution
  • Add # of Hepes to the solution
  • Add # of Potassium Chloride to the solution
  • Add # of EGTA to the solution
  • Add # of Methanesulfonic acid to the solution
  • Add distilled water until the volume is 1 L.
  • Use methanesulfonic acid to adjust the pH to 7.1-7.2. Store for up to 3 years at room temperature (22°C-24°C).
References
This online resource may be cited as follows
MLA
"Quest Calculate™ Bath Solution (Ca2+/Mg2+ Free) Preparation and Recipe." AAT Bioquest, Inc., 5 Dec. 2025, https://www.aatbio.com/resources/buffer-preparations-and-recipes/bath-solution-ca2-mg2-free.
APA
AAT Bioquest, Inc. (2025, December 5). Quest Calculate™ Bath Solution (Ca2+/Mg2+ Free) Preparation and Recipe. AAT Bioquest. https://www.aatbio.com/resources/buffer-preparations-and-recipes/bath-solution-ca2-mg2-free.
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