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Tris/EACA Buffer Preparation and Recipe

To prepare  L of Tris/EACA Buffer:
Change the value in the textbox above to scale the recipe volume

Table 1. Required components
ε-aminocaproic acid (EACA) (mw: 131.17 g/mol)1.31 g0.01 M
Tris base (mw: 121.14 g/mol)10.9 g0.09 M
  1. Prepare 800 mL of distilled water in a suitable container.
  2. Add 1.31 g of ε-aminocaproic acid (EACA) to the solution.
  3. Add 10.9 g of Tris base to the solution.
  4. Add distilled water until the volume is 1 L.

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Physiological Buffer
pH Buffering
Sample Preparation
Cell/Culture/Growth Media
Gel Electrophoresis


This online tool may be cited as follows


"Quest Calculate™ Tris/EACA Buffer Preparation and Recipe." AAT Bioquest, Inc.3 Feb2023


AAT Bioquest, Inc. (2023February 3). Quest Calculate™ Tris/EACA Buffer Preparation and Recipe. AAT Bioquest.