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IPTG Solution Preparation and Recipe

IPTG (isopropyl-beta-D-thiogalactopyranoside) is a synthetic lactose analog.

To prepare  L of IPTG Solution:
Change the value in the textbox above to scale the recipe volume

Table 1. Required components
ComponentAmountConcentration
IPTG (mw: 238.30 g/mol)200 g0.8393 M
  1. Prepare 800 mL of distilled water in a suitable container.
  2. Add 200 g of IPTG to the solution.
  3. Add distilled water until the volume is 1 L.
  4. Sterilize by passing it through a 0.22-µm disposable filter. Dispense the solution into 1-ml aliquots and store them at -20°C.

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Physiological Buffer
pH Buffering
Sample Preparation
BioAssays
Misc
Cell/Culture/Growth Media
Gel Electrophoresis


References

This online tool may be cited as follows

MLA

"Quest Calculate™ IPTG Solution Preparation and Recipe." AAT Bioquest, Inc.1 Oct2022https://www.aatbio.com/resources/buffer-preparations-and-recipes/iptg-solution.

APA

AAT Bioquest, Inc. (2022October 1). Quest Calculate™ IPTG Solution Preparation and Recipe. AAT Bioquest. https://www.aatbio.com/resources/buffer-preparations-and-recipes/iptg-solution.