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IPTG Solution Preparation and Recipe

IPTG (isopropyl-beta-D-thiogalactopyranoside) is a synthetic lactose analog.

To prepare  L of IPTG Solution:
Change the value in the textbox above to scale the recipe volume

Table 1. Required components
IPTG (mw: 238.30 g/mol)200 g0.8393 M
  1. Prepare 800 mL of distilled water in a suitable container.
  2. Add 200 g of IPTG to the solution.
  3. Add distilled water until the volume is 1 L.
  4. Sterilize by passing it through a 0.22-µm disposable filter. Dispense the solution into 1-ml aliquots and store them at -20°C.

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This online tool may be cited as follows


"Quest Calculate™ IPTG Solution Preparation and Recipe." AAT Bioquest, Inc.3 Feb2023


AAT Bioquest, Inc. (2023February 3). Quest Calculate™ IPTG Solution Preparation and Recipe. AAT Bioquest.