IPTG Solution Preparation and Recipe

IPTG (isopropyl-beta-D-thiogalactopyranoside) is a synthetic lactose analog.

To prepare

L of IPTG Solution:

Change the value in the textbox above to scale the recipe volume

Table 1. Required components

Component	Amount	Concentration
IPTG (mw: 238.3 g / mol)	#	#

Prepare 800 mL of distilled water in a suitable container.
Add # of IPTG to the solution
Add distilled water until the volume is 1 L.
Sterilize by passing it through a 0.22-µm disposable filter. Dispense the solution into 1-ml aliquots and store them at -20°C.

References

This online resource may be cited as follows

MLA	"Quest Calculate™ IPTG Solution Preparation and Recipe." AAT Bioquest, Inc., 20 Oct. 2025, https://www.aatbio.com/resources/buffer-preparations-and-recipes/iptg-solution.
APA	AAT Bioquest, Inc. (2025, October 20). Quest Calculate™ IPTG Solution Preparation and Recipe. AAT Bioquest. https://www.aatbio.com/resources/buffer-preparations-and-recipes/iptg-solution.
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