AAT Bioquest

Citrate-Phosphate Buffer (0.15 M, pH 5.0) Preparation and Recipe

A traditional buffer originally introduced in 1921. Since the Citrate-Phosphate buffer (also known as the McIlvaine buffer) only has 2 ingredients, the recipe can be adjusted to a pH range of 3-8. It is used for multiple applications in cell biology, molecular biology, and hematology.

To prepare  L of Citrate-Phosphate Buffer (0.15 M, pH 5.0):
Change the value in the textbox above to scale the recipe volume

Table 1. Required components
Sodium Phosphate Dibasic Dihydrate (mw: 177.99 g/mol)18.15 g0.102 M
Citric Acid (mw: 192.12 g/mol)9.605 g0.05 M
  1. Prepare 800 mL of distilled water in a suitable container.
  2. Add 18.15 g of Sodium Phosphate Dibasic Dihydrate to the solution.
  3. Add 9.605 g of Citric Acid to the solution.
  4. Adjust solution to final desired pH using HCl or NaOH
  5. Add distilled water until the volume is 1 L.

Physiological Buffer
pH Buffering
Sample Preparation
Cell/Culture/Growth Media
Gel Electrophoresis


This online tool may be cited as follows


"Quest Calculate™ Citrate-Phosphate Buffer (0.15 M, pH 5.0) Preparation and Recipe." AAT Bioquest, Inc.23 Jul2024https://www.aatbio.com/resources/buffer-preparations-and-recipes/citrate-phosphate-buffer-ph-5.


AAT Bioquest, Inc. (2024July 23). Quest Calculate™ Citrate-Phosphate Buffer (0.15 M, pH 5.0) Preparation and Recipe. AAT Bioquest. https://www.aatbio.com/resources/buffer-preparations-and-recipes/citrate-phosphate-buffer-ph-5.