Citrate-Phosphate Buffer (0.15 M, pH 5.0) Preparation and Recipe

A traditional buffer originally introduced in 1921. Since the Citrate-Phosphate buffer (also known as the McIlvaine buffer) only has 2 ingredients, the recipe can be adjusted to a pH range of 3-8. It is used for multiple applications in cell biology, molecular biology, and hematology.

To prepare L of Citrate-Phosphate Buffer (0.15 M, pH 5.0):
Change the value in the textbox above to scale the recipe volume

Table 1. Required components

Component	Amount	Concentration
Sodium Phosphate Dibasic Dihydrate (mw: 177.99 g/mol)	18.15 g	0.102 M
Citric Acid (mw: 192.12 g/mol)	9.605 g	0.05 M

Prepare 800 mL of distilled water in a suitable container.
Add 18.15 g of Sodium Phosphate Dibasic Dihydrate to the solution.
Add 9.605 g of Citric Acid to the solution.
Adjust solution to final desired pH using HCl or NaOH
Add distilled water until the volume is 1 L.

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Physiological Buffer

pH Buffering

Sample Preparation

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Cell/Culture/Growth Media

Gel Electrophoresis

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Citrate Buffer (0.1 M, pH 6.0)
Citrate Buffer (pH 3.0 to 6.2)
Citrate-Phosphate Buffer (0.15 M, pH 5.0)
Citrate-Phosphate Buffer (110 mM, pH 5.6)
Citrate-Phosphate Buffer (40 mM, pH 5.6)
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Potassium Phosphate Buffer (1 M, pH 6.5)
Potassium Phosphate (pH 5.8 to 8.0)
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Sodium Borate Buffer (1 M, pH 8.5)
Sodium Phosphate Solution
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TES Buffer (1 M, 7.5 pH)
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References

This online tool may be cited as follows

MLA	"Quest Calculate™ Citrate-Phosphate Buffer (0.15 M, pH 5.0) Preparation and Recipe." AAT Bioquest, Inc., 26 Apr. 2024, https://www.aatbio.com/resources/buffer-preparations-and-recipes/citrate-phosphate-buffer-ph-5.
APA	AAT Bioquest, Inc. (2024, April 26). Quest Calculate™ Citrate-Phosphate Buffer (0.15 M, pH 5.0) Preparation and Recipe. AAT Bioquest. https://www.aatbio.com/resources/buffer-preparations-and-recipes/citrate-phosphate-buffer-ph-5.
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Citrate-Phosphate Buffer (0.15 M, pH 5.0) Preparation and Recipe

References

This online tool may be cited as follows

MLA

APA