AAT Bioquest

Acetate Buffer (0.1 M, pH 5.0) Preparation and Recipe

Sodium acetate buffers are used for purification and precipitation of nucleic acids, as well as for protein crystallization and staining gels used in protein electrophoresis.

To prepare  L of Acetate Buffer (0.1 M, pH 5.0):
Change the value in the textbox above to scale the recipe volume

Table 1. Required components
Sodium Acetate (mw: 82.03 g/mol)5.772 g0.07 M
Acetic Acid (mw: 60.05 g/mol)1.778 g0.03 M
  1. Prepare 800 mL of distilled water in a suitable container.
  2. Add 5.772 g of Sodium Acetate to the solution.
  3. Add 1.778 g of Acetic Acid to the solution.
  4. Adjust solution to desired pH using 10N HCl (typically pH ≈ 5.0).
  5. Add distilled water until the volume is 1 L.

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Physiological Buffer
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This online tool may be cited as follows


"Quest Calculate™ Acetate Buffer (0.1 M, pH 5.0) Preparation and Recipe." AAT Bioquest, Inc.20 Jun2024https://www.aatbio.com/resources/buffer-preparations-and-recipes/acetate-buffer-ph-5.


AAT Bioquest, Inc. (2024June 20). Quest Calculate™ Acetate Buffer (0.1 M, pH 5.0) Preparation and Recipe. AAT Bioquest. https://www.aatbio.com/resources/buffer-preparations-and-recipes/acetate-buffer-ph-5.

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Tea Waste as a Green Method in Removing Aluminum from Drinking Water
Journal: (2022)