Acetate Buffer (0.1 M, pH 5.0) Preparation and Recipe

Sodium acetate buffers are used for purification and precipitation of nucleic acids, as well as for protein crystallization and staining gels used in protein electrophoresis.

To prepare

L of Acetate Buffer (0.1 M, pH 5.0):

Change the value in the textbox above to scale the recipe volume

Table 1. Required components

Component	Amount	Concentration
Sodium Acetate (mw: 82.03 g / mol)	#	#
Acetic Acid (mw: 60.05 g / mol)	#	#

Prepare 800 mL of distilled water in a suitable container.
Add # of Sodium Acetate to the solution
Add # of Acetic Acid to the solution
Adjust solution to desired pH using 10N HCl (typically pH ≈ 5.0).
Add distilled water until the volume is 1 L.

References

This online resource may be cited as follows

MLA	"Quest Calculate™ Acetate Buffer (0.1 M, pH 5.0) Preparation and Recipe." AAT Bioquest, Inc., 28 Oct. 2025, https://www.aatbio.com/resources/buffer-preparations-and-recipes/acetate-buffer-ph-5.
APA	AAT Bioquest, Inc. (2025, October 28). Quest Calculate™ Acetate Buffer (0.1 M, pH 5.0) Preparation and Recipe. AAT Bioquest. https://www.aatbio.com/resources/buffer-preparations-and-recipes/acetate-buffer-ph-5.
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Authors:

Calkins, Sarah