AAT Bioquest

Trizma® Buffer (pH 7.0 to 9.2) Preparation and Recipe

Trizma® is a proprietary chemical buffer used similarly to Tris buffer. It is commonly used in protein extraction for many types of IHC assays as well as blot applications. It is used in sandwich ELISA protocols for protection of the antibodies, and in electrophoresis of nucleic acids.

To prepare  L of Trizma® Buffer (pH 7.0 to 9.2):
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Current molarity:
0.1 M
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Current pH:
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Table 1. Required components
Trizma Hydrochloride (mw: 157.60 g/mol)1.011 g0.006415 M
Trizma Base (mw: 121.14 g/mol)11.337 g0.09358 M
  1. Prepare 800 mL of distilled water in a suitable container.
  2. Add 1.011 g of Trizma Hydrochloride to the solution.
  3. Add 11.337 g of Trizma Base to the solution.
  4. Add distilled water until the volume is 1 L.

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Physiological Buffer
pH Buffering
Sample Preparation
Cell/Culture/Growth Media
Gel Electrophoresis


This online tool may be cited as follows


"Quest Calculate™ Trizma® Buffer (pH 7.0 to 9.2) Preparation and Recipe." AAT Bioquest, Inc.25 May2024https://www.aatbio.com/resources/buffer-preparations-and-recipes/trizma-buffer-ph-7-2-to-9-0.


AAT Bioquest, Inc. (2024May 25). Quest Calculate™ Trizma® Buffer (pH 7.0 to 9.2) Preparation and Recipe. AAT Bioquest. https://www.aatbio.com/resources/buffer-preparations-and-recipes/trizma-buffer-ph-7-2-to-9-0.