Trizma® Buffer (pH 7.0 to 9.2) Preparation and Recipe

Trizma® is a proprietary chemical buffer used similarly to Tris buffer. It is commonly used in protein extraction for many types of IHC assays as well as blot applications. It is used in sandwich ELISA protocols for protection of the antibodies, and in electrophoresis of nucleic acids.

To prepare

L of Trizma® Buffer (pH 7.0 to 9.2):

Change the value in the textbox above to scale the recipe volume

Current molarity: 0.1	Adjust the molarity of the solution by using the slider below
Current pH: 9	Adjust the pH of the solution by using the slider below

Table 1. Required components

Component	Amount	Concentration
Trizma Hydrochloride (mw: 157.6 g / mol)	#	#
Trizma Base (mw: 121.14 g / mol)	#	#

Prepare 800 mL of distilled water in a suitable container.
Add # of Trizma Hydrochloride to the solution
Add # of Trizma Base to the solution
Add distilled water until the volume is 1 L.

References

This online resource may be cited as follows

MLA	"Quest Calculate™ Trizma® Buffer (pH 7.0 to 9.2) Preparation and Recipe." AAT Bioquest, Inc., 12 Oct. 2025, https://www.aatbio.com/resources/buffer-preparations-and-recipes/trizma-buffer-ph-7-2-to-9-0.
APA	AAT Bioquest, Inc. (2025, October 12). Quest Calculate™ Trizma® Buffer (pH 7.0 to 9.2) Preparation and Recipe. AAT Bioquest. https://www.aatbio.com/resources/buffer-preparations-and-recipes/trizma-buffer-ph-7-2-to-9-0.
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