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Trizma® Buffer (pH 7.0 to 9.2) Preparation and Recipe

Trizma® is a proprietary chemical buffer used similarly to Tris buffer. It is commonly used in protein extraction for many types of IHC assays as well as blot applications. It is used in sandwich ELISA protocols for protection of the antibodies, and in electrophoresis of nucleic acids.

To prepare  L of Trizma® Buffer (pH 7.0 to 9.2):
Change the value in the textbox above to scale the recipe volume

Current molarity:
0.1 M
Adjust the molarity of the solution by using the slider below
Current pH:
9
Adjust the pH of the solution by using the slider below

Table 1. Required components
ComponentAmountConcentration
Trizma Hydrochloride (mw: 157.60 g/mol)1.011 g0.006415 M
Trizma Base (mw: 121.14 g/mol)11.337 g0.09358 M
  1. Prepare 800 mL of distilled water in a suitable container.
  2. Add 1.011 g of Trizma Hydrochloride to the solution.
  3. Add 11.337 g of Trizma Base to the solution.
  4. Add distilled water until the volume is 1 L.

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Physiological Buffer
pH Buffering
Sample Preparation
BioAssays
Misc
Cell/Culture/Growth Media
Gel Electrophoresis


References

This online tool may be cited as follows

MLA

"Quest Calculate™ Trizma® Buffer (pH 7.0 to 9.2) Preparation and Recipe." AAT Bioquest, Inc.1 Oct2022https://www.aatbio.com/resources/buffer-preparations-and-recipes/trizma-buffer-ph-7-2-to-9-0.

APA

AAT Bioquest, Inc. (2022October 1). Quest Calculate™ Trizma® Buffer (pH 7.0 to 9.2) Preparation and Recipe. AAT Bioquest. https://www.aatbio.com/resources/buffer-preparations-and-recipes/trizma-buffer-ph-7-2-to-9-0.