Citrate-Phosphate Buffer (40 mM, pH 5.6) Preparation and Recipe

A traditional buffer originally introduced in 1921. Since the Citrate-Phosphate buffer (also known as the McIlvaine buffer) only has 2 ingredients, the recipe can be adjusted to a pH range of 3-8. It is used for multiple applications in cell biology, molecular biology, and hematology.

To prepare L of Citrate-Phosphate Buffer (40 mM, pH 5.6):
Change the value in the textbox above to scale the recipe volume

Table 1. Required components

Component	Amount	Concentration
Sodium phosphate dibasic (anhydrous) (mw: 141.96 g/mol)	2.82 g	0.01986 M
Citric Acid (mw: 192.12 g/mol)	4.2 g	0.02186 M

Prepare 800 mL of distilled water in a suitable container.
Add 2.82 g of Sodium phosphate dibasic (anhydrous) to the solution.
Add 4.2 g of Citric Acid to the solution.
Add distilled water until the volume is 1 L.
Sterilize by autoclaving. (The final solution contains 20 mM Na2HPO4.)

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Physiological Buffer

pH Buffering

Sample Preparation

BioAssays

Misc

Cell/Culture/Growth Media

Gel Electrophoresis

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Extracellular Saline (1x, pH 7.3)
HBSS (Hank's Balanced Salt Solution)
HEPES Buffer (1 M, 7.5 pH)
HHBS (Hank's Buffer with Hepes)
PBS (Phosphate Buffered Saline) (1X, pH 7.4)
PBS with Ca2+and Mg2+ (D-PBS)
Tyrode's Solution, Acidic

Name ▲ ▼
ACES Buffer (0.1 M, 6.7 pH)
Acetate Buffer (0.1 M, pH 5.0)
Acetate Buffer (pH 3.6 to 5.6)
ADA Buffer (0.5 M, 6.6 pH)
Ammonium Acetate
Ammonium Bicarbonate (50 mM, pH 7.8)
Ammonium Sulfate, Saturated
BES-Buffered Saline (2X) (0.05 M, pH 6.95)
Bicine (1 M, pH 8.26)
Bis-Tris Buffer (1 M, 6.5 pH)
Borate Buffer
CAPS (0.5 M, pH 10.4)
Carbonate-Bicarbonate Buffer (pH 9.2 to 10.6)
CHES (0.5 M, pH 9.5)
Citrate Buffer (0.1 M, pH 6.0)
Citrate Buffer (pH 3.0 to 6.2)
Citrate-Phosphate Buffer (0.15 M, pH 5.0)
Citrate-Phosphate Buffer (110 mM, pH 5.6)
Citrate-Phosphate Buffer (40 mM, pH 5.6)
Diethanolamine (1 M, pH 9.8)
EBSS (magnesium, calcium, phenol red) (pH 7.0)
Glycine (0.1 M, pH 2.2)
Glycine-HCl Buffer (0.1 M, pH 3.0)
Glycine-Sodium Hydroxide Buffer (0.08 M, pH 10)
HEPPSO (1 M, pH 7.85)
Hydrochloric Acid-Potassium Chloride Buffer (0.1 M, pH 2.0)
Imidazole-HCl Buffer (0.05 M, pH 7.0)
Maleic Acid (1 M, pH 7.5)
MES (0.5 M, pH 6)
MOPS Buffer (10X) (0.2 M, pH 7)
MOPSO Buffer (0.5 M, 6.9 pH)
Phosphate Buffer (pH 5.8 to 7.4)
PIPES Buffer (1 M, 6.8 pH)
Potassium Phosphate Buffer (1 M, pH 6.5)
Potassium Phosphate (pH 5.8 to 8.0)
Sodium Acetate (3 M, pH 5.2)
Sodium Borate Buffer (1 M, pH 8.5)
Sodium Phosphate Solution
SSC buffer (20X) (3 M, pH 7)
TAE (1 M, pH 8.6)
TB Buffer
TBS (1 M, pH 7.4)
TES Buffer (1 M, 7.5 pH)
Tricine (1 M, pH 8.05)
Tris Buffer (1 M, pH 7.2)
Tris-Buffered Saline (TBS, 0.1 M)
Tris-citrate Buffer (pH 8.0)
Trizma® Buffer (pH 7.0 to 9.2)

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TE Buffer 10X
Trichloroacetic Acid (TCA)

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Anesthetic (MS222) Solution
Bradford Stock Solution
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Immunofluorescence Quenching Solution
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Nuclear Fast Red Solution
Pyronin Y (PY) Solution
SSC (Northern Blot, 20x)
SSPE (Blot, 20x, pH 7.4)

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Dithiothreitol (DTT) (1 M)
Sodium Iodide/Sodium Sulfite Solution

Name ▲ ▼
Bath Solution (Ca2+/Mg2+ Free)
Calcium Chloride Stock Solution
Carbenicillin Solution
Chelation Medium
Digestion Medium
Embryonic Medium
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Modified Barth's Saline (MBS)
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Yeast Two Hybrid (Y2H) Media, Amino Acid Dropout Mixes

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Acrylamide Stock Solution
Electrode Buffer
Electrophoretic Transfer Buffer (20x)
Imidazole-SDS Solution
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Tris/EACA Buffer
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References

This online tool may be cited as follows

MLA	"Quest Calculate™ Citrate-Phosphate Buffer (40 mM, pH 5.6) Preparation and Recipe." AAT Bioquest, Inc., 26 Apr. 2024, https://www.aatbio.com/resources/buffer-preparations-and-recipes/citrate-phosphate-buffer-40-mm-ph-5-6.
APA	AAT Bioquest, Inc. (2024, April 26). Quest Calculate™ Citrate-Phosphate Buffer (40 mM, pH 5.6) Preparation and Recipe. AAT Bioquest. https://www.aatbio.com/resources/buffer-preparations-and-recipes/citrate-phosphate-buffer-40-mm-ph-5-6.
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Citrate-Phosphate Buffer (40 mM, pH 5.6) Preparation and Recipe

References

This online tool may be cited as follows

MLA

APA