Maleic Acid (1 M, pH 7.5) Preparation and Recipe

Maleic acid buffer is commonly used for Southern blot preparation and as a washing buffer in numerous applications involving nucleic acids. It has a shelf life of 6 months to a year.

To prepare

L of Maleic Acid (1 M, pH 7.5):

Change the value in the textbox above to scale the recipe volume

Table 1. Required components

Component	Amount	Concentration
Maleic Acid (mw: 116.07 g / mol)	#	#
Sodium chloride (mw: 58.44 g / mol)	#	#
Sodium Hydroxide (mw: 40 g / mol)	#	#

Prepare 800 mL of dH2O in a suitable container.
Add # of Maleic Acid to the solution
Add # of Sodium chloride to the solution
Add # of Sodium Hydroxide to the solution
Add dH2O until the volume is 1 L.
Sterilize by autoclaving at 15 psi for 20 minutes or by filtration. Store at room temperature. For washing applications, add 0.2% Tween-20 if desired.

References

This online resource may be cited as follows

MLA	"Quest Calculate™ Maleic Acid (1 M, pH 7.5) Preparation and Recipe." AAT Bioquest, Inc., 22 Sep. 2025, https://www.aatbio.com/resources/buffer-preparations-and-recipes/maleic-acid-ph-7-5.
APA	AAT Bioquest, Inc. (2025, September 22). Quest Calculate™ Maleic Acid (1 M, pH 7.5) Preparation and Recipe. AAT Bioquest. https://www.aatbio.com/resources/buffer-preparations-and-recipes/maleic-acid-ph-7-5.
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