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Trichloroacetic Acid (TCA) Preparation and Recipe

TCA (trichloroacetic acid) is used for protein and DNA precipitation.

To prepare  L of Trichloroacetic Acid (TCA):
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Table 1. Required components
Trichloroacetic acid (TCA) (mw: 163.39 g/mol)1101.32 g6.7404 M
  1. Prepare 800 mL of distilled water in a suitable container.
  2. Add 1101.32 g of Trichloroacetic acid (TCA) to the solution.
  3. Add distilled water until the volume is 1 L.
  4. The resulting solution will contain 100% (w/v) TCA.

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This online tool may be cited as follows


"Quest Calculate™ Trichloroacetic Acid (TCA) Preparation and Recipe." AAT Bioquest, Inc.2 Feb2023


AAT Bioquest, Inc. (2023February 2). Quest Calculate™ Trichloroacetic Acid (TCA) Preparation and Recipe. AAT Bioquest.