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TB Buffer Preparation and Recipe
To prepareL of TB Buffer:
Change the value in the textbox above to scale the recipe volume
Table 1. Required components
| Component | Amount | Concentration |
|---|---|---|
| EDTA (mw: 292.24 g / mol) | # | # |
| Potassium Chloride (mw: 74.55 g / mol) | # | # |
| Sodium chloride (mw: 58.44 g / mol) | # | # |
| Phenylmethylsulfonyl fluoride (PMSF) (mw: 174.19 g / mol) | # | # |
| Tris-hydrochloride (mw: 157.6 g / mol) | # | # |
- Prepare 800 mL of distilled water in a suitable container.
- Add # of EDTA to the solution
- Add # of Potassium Chloride to the solution
- Add # of Sodium chloride to the solution
- Add # of Phenylmethylsulfonyl fluoride (PMSF) to the solution
- Add # of Tris-hydrochloride to the solution
- Adjust pH to 6.8.
- Add distilled water until the volume is 1 L.
- Sterilize by filtration.
References
This online resource may be cited as follows
MLA | "Quest Calculate™ TB Buffer Preparation and Recipe." AAT Bioquest, Inc., 16 Oct. 2025, https://www.aatbio.com/resources/buffer-preparations-and-recipes/tb-buffer. | |
APA | AAT Bioquest, Inc. (2025, October 16). Quest Calculate™ TB Buffer Preparation and Recipe. AAT Bioquest. https://www.aatbio.com/resources/buffer-preparations-and-recipes/tb-buffer. | |
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