AAT Bioquest

TB Buffer Preparation and Recipe

To prepare  L of TB Buffer:
Change the value in the textbox above to scale the recipe volume

Table 1. Required components
EDTA (mw: 292.24 g/mol)0.37 g0.001 M
Potassium Chloride (mw: 74.55 g/mol)13.64 g0.183 M
Sodium chloride (mw: 58.44 g/mol)2.74 g0.047 M
Phenylmethylsulfonyl fluoride (PMSF) (mw: 174.19 g/mol)0.17 g0.001 M
Tris-hydrochloride (mw: 157.60 g/mol)1.21 g0.01 M
  1. Prepare 800 mL of distilled water in a suitable container.
  2. Add 0.37 g of EDTA to the solution.
  3. Add 13.64 g of Potassium Chloride to the solution.
  4. Add 2.74 g of Sodium chloride to the solution.
  5. Add 0.17 g of Phenylmethylsulfonyl fluoride (PMSF) to the solution.
  6. Add 1.21 g of Tris-hydrochloride to the solution.
  7. Adjust pH to 6.8.
  8. Add distilled water until the volume is 1 L.
  9. Sterilize by filtration.

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Physiological Buffer
pH Buffering
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This online tool may be cited as follows


"Quest Calculate™ TB Buffer Preparation and Recipe." AAT Bioquest, Inc.23 Jun2024https://www.aatbio.com/resources/buffer-preparations-and-recipes/tb-buffer.


AAT Bioquest, Inc. (2024June 23). Quest Calculate™ TB Buffer Preparation and Recipe. AAT Bioquest. https://www.aatbio.com/resources/buffer-preparations-and-recipes/tb-buffer.