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Tris-citrate Buffer (pH 8.0) Preparation and Recipe

Tris-citrate buffer can be used for electrophoresis of bcterial enzymes.

To prepare  L of Tris-citrate Buffer (pH 8.0):
Change the value in the textbox above to scale the recipe volume

Table 1. Required components
Citric Acid (mw: 192.12 g/mol)16 g0.08325 M
Tris (mw: 121.14 g/mol)40.2 g0.3318 M
  1. Prepare 800 mL of distilled water in a suitable container.
  2. Add 16 g of Citric Acid to the solution.
  3. Add 40.2 g of Tris to the solution.
  4. Add distilled water until the volume is 1 L.

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Physiological Buffer
pH Buffering
Sample Preparation
Cell/Culture/Growth Media
Gel Electrophoresis


This online tool may be cited as follows


"Quest Calculate™ Tris-citrate Buffer (pH 8.0) Preparation and Recipe." AAT Bioquest, Inc.3 Feb2023


AAT Bioquest, Inc. (2023February 3). Quest Calculate™ Tris-citrate Buffer (pH 8.0) Preparation and Recipe. AAT Bioquest.