Tris-citrate Buffer (pH 8.0) Preparation and Recipe
Tris-citrate buffer can be used for electrophoresis of bcterial enzymes.
To prepare L of Tris-citrate Buffer (pH 8.0):
Change the value in the textbox above to scale the recipe volume
Change the value in the textbox above to scale the recipe volume
Table 1. Required components
Component | Amount | Concentration |
Citric Acid (mw: 192.12 g/mol) | 16 g | 0.08325 M |
Tris (mw: 121.14 g/mol) | 40.2 g | 0.3318 M |
- Prepare 800 mL of distilled water in a suitable container.
- Add 16 g of Citric Acid to the solution.
- Add 40.2 g of Tris to the solution.
- Add distilled water until the volume is 1 L.
Physiological Buffer
pH Buffering
Sample Preparation
BioAssays
Misc
Cell/Culture/Growth Media
Gel Electrophoresis
References
This online tool may be cited as follows
MLA | "Quest Calculate™ Tris-citrate Buffer (pH 8.0) Preparation and Recipe." AAT Bioquest, Inc., 12 Oct. 2024, https://www.aatbio.com/resources/buffer-preparations-and-recipes/tris-citrate-buffer-ph-8-0. | |
APA | AAT Bioquest, Inc. (2024, October 12). Quest Calculate™ Tris-citrate Buffer (pH 8.0) Preparation and Recipe. AAT Bioquest. https://www.aatbio.com/resources/buffer-preparations-and-recipes/tris-citrate-buffer-ph-8-0. | |
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