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Tris-citrate Buffer (pH 8.0) Preparation and Recipe
Tris-citrate buffer can be used for electrophoresis of bcterial enzymes.
To prepareL of Tris-citrate Buffer (pH 8.0):
Change the value in the textbox above to scale the recipe volume
Table 1. Required components
| Component | Amount | Concentration |
|---|---|---|
| Citric Acid (mw: 192.12 g / mol) | # | # |
| Tris (mw: 121.14 g / mol) | # | # |
- Prepare 800 mL of distilled water in a suitable container.
- Add # of Citric Acid to the solution
- Add # of Tris to the solution
- Add distilled water until the volume is 1 L.
References
This online resource may be cited as follows
MLA | "Quest Calculate™ Tris-citrate Buffer (pH 8.0) Preparation and Recipe." AAT Bioquest, Inc., 30 Oct. 2025, https://www.aatbio.com/resources/buffer-preparations-and-recipes/tris-citrate-buffer-ph-8-0. | |
APA | AAT Bioquest, Inc. (2025, October 30). Quest Calculate™ Tris-citrate Buffer (pH 8.0) Preparation and Recipe. AAT Bioquest. https://www.aatbio.com/resources/buffer-preparations-and-recipes/tris-citrate-buffer-ph-8-0. | |
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