AAT Bioquest

Gey's Balanced Salt Solution Preparation and Recipe

Gey’s Balanced Salt Solution can be used for cell suspension or as washing solution.

To prepare  L of Gey's Balanced Salt Solution:
Change the value in the textbox above to scale the recipe volume

Table 1. Required components
Calcium chloride dihydrate (mw: 147.01 g/mol)0.22 g0.001496 M
Potassium Chloride (mw: 74.55 g/mol)0.37 g0.004963 M
Potassium Phosphate Monobasic (mw: 136.09 g/mol)0.03 g0.0002204 M
Magnesium Chloride Hexahydrate (mw: 203.30 g/mol)0.21 g0.001032 M
Magnesium Sulfate Heptahydrate (mw: 246.47 g/mol)0.07 g0.000284 M
Sodium chloride (mw: 58.44 g/mol)8 g0.1368 M
Sodium bicarbonate (mw: 84.01 g/mol)0.227 g0.002702 M
Sodium Phosphate Dibasic (mw: 141.96 g/mol)0.12 g0.0008453 M
D-glucose (mw: 180.16 g/mol)1 g0.00555 M
  1. Prepare 800 mL of distilled water in a suitable container.
  2. Add 0.22 g of Calcium chloride dihydrate to the solution.
  3. Add 0.37 g of Potassium Chloride to the solution.
  4. Add 0.03 g of Potassium Phosphate Monobasic to the solution.
  5. Add 0.21 g of Magnesium Chloride Hexahydrate to the solution.
  6. Add 0.07 g of Magnesium Sulfate Heptahydrate to the solution.
  7. Add 8 g of Sodium chloride to the solution.
  8. Add 0.227 g of Sodium bicarbonate to the solution.
  9. Add 0.12 g of Sodium Phosphate Dibasic to the solution.
  10. Add 1 g of D-glucose to the solution.
  11. Add distilled water until the volume is 1 L.
  12. Sterile filter and store in 500-mL aliquots at 4?C.

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Physiological Buffer
pH Buffering
Sample Preparation
Cell/Culture/Growth Media
Gel Electrophoresis


This online tool may be cited as follows


"Quest Calculate™ Gey's Balanced Salt Solution Preparation and Recipe." AAT Bioquest, Inc.20 Jun2024https://www.aatbio.com/resources/buffer-preparations-and-recipes/gey-s-balanced-salt-solution.


AAT Bioquest, Inc. (2024June 20). Quest Calculate™ Gey's Balanced Salt Solution Preparation and Recipe. AAT Bioquest. https://www.aatbio.com/resources/buffer-preparations-and-recipes/gey-s-balanced-salt-solution.