AAT Bioquest

Extracellular Saline (1x, pH 7.3) Preparation and Recipe

To prepare  L of Extracellular Saline (1x, pH 7.3):
Change the value in the textbox above to scale the recipe volume

Table 1. Required components
Sodium chloride (mw: 58.44 g/mol)8.182 g0.14 M
Potassium Chloride (mw: 74.55 g/mol)0.402 g0.0054 M
Calcium Chloride (mw: 110.98 g/mol)0.294 g0.002 M
Magnesium sulfate (mw: 120.37 g/mol)1.204 g0.01 M
Hepes (mw: 238.30 g/mol)1.191 g0.005 M
Glucose (mw: 180.16 g/mol)1.802 g0.01 M
  1. Prepare 800 mL of distilled water in a suitable container.
  2. Add 8.182 g of Sodium chloride to the solution.
  3. Add 0.402 g of Potassium Chloride to the solution.
  4. Add 0.294 g of Calcium Chloride to the solution.
  5. Add 1.204 g of Magnesium sulfate to the solution.
  6. Add 1.191 g of Hepes to the solution.
  7. Add 1.802 g of Glucose to the solution.
  8. Add distilled water until the volume is 1 L.

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Physiological Buffer
pH Buffering
Sample Preparation
Cell/Culture/Growth Media
Gel Electrophoresis


This online tool may be cited as follows


"Quest Calculate™ Extracellular Saline (1x, pH 7.3) Preparation and Recipe." AAT Bioquest, Inc.20 Apr2024https://www.aatbio.com/resources/buffer-preparations-and-recipes/extracellular-saline-rnase-free-1x-ph-7-3.


AAT Bioquest, Inc. (2024April 20). Quest Calculate™ Extracellular Saline (1x, pH 7.3) Preparation and Recipe. AAT Bioquest. https://www.aatbio.com/resources/buffer-preparations-and-recipes/extracellular-saline-rnase-free-1x-ph-7-3.