Extracellular Saline (1x, pH 7.3) Preparation and Recipe

Table 1. Required components

Component	Amount	Concentration
Sodium chloride (mw: 58.44 g/mol)	8.182 g	0.14 M
Potassium Chloride (mw: 74.55 g/mol)	0.402 g	0.0054 M
Calcium Chloride (mw: 110.98 g/mol)	0.294 g	0.002 M
Magnesium sulfate (mw: 120.37 g/mol)	1.204 g	0.01 M
Hepes (mw: 238.30 g/mol)	1.191 g	0.005 M
Glucose (mw: 180.16 g/mol)	1.802 g	0.01 M

1. Prepare 800 mL of distilled water in a suitable container.
2. Add 8.182 g of Sodium chloride to the solution.
3. Add 0.402 g of Potassium Chloride to the solution.
4. Add 0.294 g of Calcium Chloride to the solution.
5. Add 1.204 g of Magnesium sulfate to the solution.
6. Add 1.191 g of Hepes to the solution.
7. Add 1.802 g of Glucose to the solution.
8. Add distilled water until the volume is 1 L.

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