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Extracellular Saline (1x, pH 7.3) Preparation and Recipe
To prepareL of Extracellular Saline (1x, pH 7.3):
Change the value in the textbox above to scale the recipe volume
Table 1. Required components
| Component | Amount | Concentration |
|---|---|---|
| Sodium chloride (mw: 58.44 g / mol) | # | # |
| Potassium Chloride (mw: 74.55 g / mol) | # | # |
| Calcium Chloride (mw: 110.98 g / mol) | # | # |
| Magnesium sulfate (mw: 120.37 g / mol) | # | # |
| Hepes (mw: 238.3 g / mol) | # | # |
| Glucose (mw: 180.16 g / mol) | # | # |
- Prepare 800 mL of distilled water in a suitable container.
- Add # of Sodium chloride to the solution
- Add # of Potassium Chloride to the solution
- Add # of Calcium Chloride to the solution
- Add # of Magnesium sulfate to the solution
- Add # of Hepes to the solution
- Add # of Glucose to the solution
- Add distilled water until the volume is 1 L.
References
This online resource may be cited as follows
MLA | "Quest Calculate™ Extracellular Saline (1x, pH 7.3) Preparation and Recipe." AAT Bioquest, Inc., 7 Nov. 2025, https://www.aatbio.com/resources/buffer-preparations-and-recipes/extracellular-saline-rnase-free-1x-ph-7-3. | |
APA | AAT Bioquest, Inc. (2025, November 7). Quest Calculate™ Extracellular Saline (1x, pH 7.3) Preparation and Recipe. AAT Bioquest. https://www.aatbio.com/resources/buffer-preparations-and-recipes/extracellular-saline-rnase-free-1x-ph-7-3. | |
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