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DEAE-dextran Preparation and Recipe

To prepare  L of DEAE-dextran:
Change the value in the textbox above to scale the recipe volume

Table 1. Required components
ComponentAmountConcentration
DEAE-dextran (mw: 500000 g/mol)50 g0.0001 M
  1. Prepare 800 mL of distilled water in a suitable container.
  2. Add 50 g of DEAE-dextran to the solution.
  3. Add distilled water until the volume is 1 L.
  4. Sterilize the solution by autoclaving for 20 minutes at 15 psi (1.05 kg/cm2) on liquid cycle. Autoclaving also assists dissolution of the polymer. The molecular weight of the DEAE-dextran originally used for transfection was >2 x 106. Although this material is no longer available commercially, it is still occasionally found in chemical storerooms. The older batches of higher-molecular-weight DEAE-dextran are more efficient for transfection than the lower-molecular-weight polymers currently available.

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Physiological Buffer
pH Buffering
Sample Preparation
BioAssays
Misc
Cell/Culture/Growth Media
Gel Electrophoresis


References

This online tool may be cited as follows

MLA

"Quest Calculate™ DEAE-dextran Preparation and Recipe." AAT Bioquest, Inc.1 Oct2022https://www.aatbio.com/resources/buffer-preparations-and-recipes/deae-dextran.

APA

AAT Bioquest, Inc. (2022October 1). Quest Calculate™ DEAE-dextran Preparation and Recipe. AAT Bioquest. https://www.aatbio.com/resources/buffer-preparations-and-recipes/deae-dextran.