DEAE-dextran Preparation and Recipe

To prepare

L of DEAE-dextran:

Change the value in the textbox above to scale the recipe volume

Table 1. Required components

Component	Amount	Concentration
DEAE-dextran (mw: 500000 g / mol)	#	#

Prepare 800 mL of distilled water in a suitable container.
Add # of DEAE-dextran to the solution
Add distilled water until the volume is 1 L.
Sterilize the solution by autoclaving for 20 minutes at 15 psi (1.05 kg/cm2) on liquid cycle. Autoclaving also assists dissolution of the polymer. The molecular weight of the DEAE-dextran originally used for transfection was >2 x 106. Although this material is no longer available commercially, it is still occasionally found in chemical storerooms. The older batches of higher-molecular-weight DEAE-dextran are more efficient for transfection than the lower-molecular-weight polymers currently available.

References

This online resource may be cited as follows

MLA	"Quest Calculate™ DEAE-dextran Preparation and Recipe." AAT Bioquest, Inc., 16 Oct. 2025, https://www.aatbio.com/resources/buffer-preparations-and-recipes/deae-dextran.
APA	AAT Bioquest, Inc. (2025, October 16). Quest Calculate™ DEAE-dextran Preparation and Recipe. AAT Bioquest. https://www.aatbio.com/resources/buffer-preparations-and-recipes/deae-dextran.
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