DEAE-dextran Preparation and Recipe

Table 1. Required components

Component	Amount	Concentration
DEAE-dextran (mw: 500000 g/mol)	50 g	0.0001 M

1. Prepare 800 mL of distilled water in a suitable container.
2. Add 50 g of DEAE-dextran to the solution.
3. Add distilled water until the volume is 1 L.
4. Sterilize the solution by autoclaving for 20 minutes at 15 psi (1.05 kg/cm2) on liquid cycle. Autoclaving also assists dissolution of the polymer. The molecular weight of the DEAE-dextran originally used for transfection was >2 x 106. Although this material is no longer available commercially, it is still occasionally found in chemical storerooms. The older batches of higher-molecular-weight DEAE-dextran are more efficient for transfection than the lower-molecular-weight polymers currently available.

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