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Carbonate-Bicarbonate Buffer (pH 9.2 to 10.6) Preparation and Recipe

Commonly used for various immunoassay applications and for many protein and antibody conjugation procedures, including sandwich ELISA, which require experimental surface coatings. Carbonate-bicarbonate buffer is used extensively in molecular and cell biology, biochemistry, and in the medical field, where it is the most commonly used small intestine buffer. It has good buffering capacity and is easy to prepare, with excellent shelf life.

To prepare  L of Carbonate-Bicarbonate Buffer (pH 9.2 to 10.6):
Change the value in the textbox above to scale the recipe volume

Current molarity:
0.1 M
Adjust the molarity of the solution by using the slider below
Current pH:
10.6
Adjust the pH of the solution by using the slider below

Table 1. Required components
ComponentAmountConcentration
Sodium bicarbonate (mw: 84.01 g/mol)1.05 g0.0125 M
Sodium carbonate (anhydrous) (mw: 105.99 g/mol)9.274 g0.0875 M
  1. Prepare 800 mL of distilled water in a suitable container.
  2. Add 1.05 g of Sodium bicarbonate to the solution.
  3. Add 9.274 g of Sodium carbonate (anhydrous) to the solution.
  4. Add distilled water until the volume is 1 L.

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Physiological Buffer
pH Buffering
Sample Preparation
BioAssays
Misc
Cell/Culture/Growth Media
Gel Electrophoresis


References

This online tool may be cited as follows

MLA

"Quest Calculate™ Carbonate-Bicarbonate Buffer (pH 9.2 to 10.6) Preparation and Recipe." AAT Bioquest, Inc.10 Aug2022https://www.aatbio.com/resources/buffer-preparations-and-recipes/carbonate-bicarbonate-buffer-ph-9-2-to-10-6.

APA

AAT Bioquest, Inc. (2022August 10). Quest Calculate™ Carbonate-Bicarbonate Buffer (pH 9.2 to 10.6) Preparation and Recipe. AAT Bioquest. https://www.aatbio.com/resources/buffer-preparations-and-recipes/carbonate-bicarbonate-buffer-ph-9-2-to-10-6.