AAT Bioquest

Carbonate-Bicarbonate Buffer (pH 9.2 to 10.6) Preparation and Recipe

Commonly used for various immunoassay applications and for many protein and antibody conjugation procedures, including sandwich ELISA, which require experimental surface coatings. Carbonate-bicarbonate buffer is used extensively in molecular and cell biology, biochemistry, and in the medical field, where it is the most commonly used small intestine buffer. It has good buffering capacity and is easy to prepare, with excellent shelf life.

To prepare  L of Carbonate-Bicarbonate Buffer (pH 9.2 to 10.6):
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Current molarity:
0.1 M
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Current pH:
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Table 1. Required components
Sodium bicarbonate (mw: 84.01 g/mol)1.05 g0.0125 M
Sodium carbonate (anhydrous) (mw: 105.99 g/mol)9.274 g0.0875 M
  1. Prepare 800 mL of distilled water in a suitable container.
  2. Add 1.05 g of Sodium bicarbonate to the solution.
  3. Add 9.274 g of Sodium carbonate (anhydrous) to the solution.
  4. Add distilled water until the volume is 1 L.

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Physiological Buffer
pH Buffering
Sample Preparation
Cell/Culture/Growth Media
Gel Electrophoresis


This online tool may be cited as follows


"Quest Calculate™ Carbonate-Bicarbonate Buffer (pH 9.2 to 10.6) Preparation and Recipe." AAT Bioquest, Inc.25 May2024https://www.aatbio.com/resources/buffer-preparations-and-recipes/carbonate-bicarbonate-buffer-ph-9-2-to-10-6.


AAT Bioquest, Inc. (2024May 25). Quest Calculate™ Carbonate-Bicarbonate Buffer (pH 9.2 to 10.6) Preparation and Recipe. AAT Bioquest. https://www.aatbio.com/resources/buffer-preparations-and-recipes/carbonate-bicarbonate-buffer-ph-9-2-to-10-6.