Carbonate-Bicarbonate Buffer (pH 9.2 to 10.6) Preparation and Recipe
Commonly used for various immunoassay applications and for many protein and antibody conjugation procedures, including sandwich ELISA, which require experimental surface coatings. Carbonate-bicarbonate buffer is used extensively in molecular and cell biology, biochemistry, and in the medical field, where it is the most commonly used small intestine buffer. It has good buffering capacity and is easy to prepare, with excellent shelf life.
To prepare L of Carbonate-Bicarbonate Buffer (pH 9.2 to 10.6):
Change the value in the textbox above to scale the recipe volume
Change the value in the textbox above to scale the recipe volume
Current molarity: 0.1 M | Adjust the molarity of the solution by using the slider below |
Current pH: 10.6 | Adjust the pH of the solution by using the slider below |
Table 1. Required components
Component | Amount | Concentration |
Sodium bicarbonate (mw: 84.01 g/mol) | 1.05 g | 0.0125 M |
Sodium carbonate (anhydrous) (mw: 105.99 g/mol) | 9.274 g | 0.0875 M |
- Prepare 800 mL of distilled water in a suitable container.
- Add 1.05 g of Sodium bicarbonate to the solution.
- Add 9.274 g of Sodium carbonate (anhydrous) to the solution.
- Add distilled water until the volume is 1 L.
Physiological Buffer
pH Buffering
Sample Preparation
BioAssays
Misc
Cell/Culture/Growth Media
Gel Electrophoresis
References
This online tool may be cited as follows
MLA | "Quest Calculate™ Carbonate-Bicarbonate Buffer (pH 9.2 to 10.6) Preparation and Recipe." AAT Bioquest, Inc., 11 Dec. 2024, https://www.aatbio.com/resources/buffer-preparations-and-recipes/carbonate-bicarbonate-buffer-ph-9-2-to-10-6. | |
APA | AAT Bioquest, Inc. (2024, December 11). Quest Calculate™ Carbonate-Bicarbonate Buffer (pH 9.2 to 10.6) Preparation and Recipe. AAT Bioquest. https://www.aatbio.com/resources/buffer-preparations-and-recipes/carbonate-bicarbonate-buffer-ph-9-2-to-10-6. | |
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This online tool has been cited in 1 publications, including
Simultaneous Determination of Lidocaine and Bupivacaine in Human Saliva Using Gas Chromatography-Mass Spectrometry
Authors:
Journal: Arab Journal of Forensic Sciences \& Forensic Medicine (2024): 19--28
Authors:
Journal: Arab Journal of Forensic Sciences \& Forensic Medicine (2024): 19--28