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Citrate Buffer (pH 3.0 to 6.2) Preparation and Recipe
Citrate buffers can be used for RNA isolation, due to its ability to prevent base hydrolysis. The buffer is also used for antigen detection by breaking cross-links between antigens and any substances in its fixation medium. Citrate buffer is a popular choice for immunofluorescent staining applications, as it can aid in stain intensity without contributing to background signal. It is a popular choice for biological research and clinical applications, including pathology, and has had an increasing role in the medical field, as it has been shown to mildly improve dialysis efficiency. It has a shelf life of up to 3 months at room temperature.

Available for purchase here.
To prepare
L of Citrate Buffer (pH 3.0 to 6.2):
Change the value in the textbox above to scale the recipe volume
Current molarity:
0.1
Adjust the molarity of the solution by using the slider below
Current pH:
6.2
Adjust the pH of the solution by using the slider below
Table 1. Required components
ComponentAmountConcentration
Sodium Citrate dihydrate (mw: 294.1 g / mol)##
Citric Acid (mw: 192.12 g / mol)##
  • Prepare 800 mL of distilled water in a suitable container.
  • Add # of Sodium Citrate dihydrate to the solution
  • Add # of Citric Acid to the solution
  • Adjust solution to final desired pH using HCl or NaOH
  • Add distilled water until the volume is 1 L.


References
This online resource may be cited as follows
MLA
"Quest CalculateCitrate Buffer (pH 3.0 to 6.2) Preparation and Recipe." AAT Bioquest, Inc.9 Oct2025https://www.aatbio.com/resources/buffer-preparations-and-recipes/citrate-buffer-ph-3-to-6-2.
APA
AAT Bioquest, Inc. (2025October 9). Quest CalculateCitrate Buffer (pH 3.0 to 6.2) Preparation and Recipe. AAT Bioquest. https://www.aatbio.com/resources/buffer-preparations-and-recipes/citrate-buffer-ph-3-to-6-2.
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This online resource has been cited in 2 publications, including
Influence of Stirring Speed on Glucose and Ethanol Production in Simultaneous Saccharification and Fermentation Process
Authors: 
Palniandy, T and Nafsun, AI and Jamil, N Mohd and Herz, F and Azmi, NAA and Zaki, MH Muhammad
Journal: 
Journal of Chemical Engineering and Industrial Biotechnology (2022)