Citrate Buffer (pH 3.0 to 6.2) recipe and preparation
Citrate buffers can be used for RNA isolation, thanks to its ability to prevent base hydrolysis. It also finds use in antigen detection by breaking cross-links between antigens and any substances in its fixation medium.
To prepare L of Citrate Buffer (pH 3.0 to 6.2): Change the value in the textbox above to scale the recipe volume
Current molarity: 0.1 M
Adjust the molarity of the solution by using the slider below
Adjust the pH of the solution by using the slider below
Table 1. Required components
Sodium Citrate dihydrate (mw: 294 g/mol)
Citric Acid (mw: 192.1 g/mol)
Prepare 800 mL of distilled water in a suitable container.
Add 24.087 g of Sodium Citrate dihydrate to the solution.
Add 3.471 g of Citric Acid to the solution.
Adjust solution to final desired pH using HCl or NaOH