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Citrate Buffer (pH 3.0 to 6.2) Preparation and Recipe

Citrate buffers can be used for RNA isolation, due to its ability to prevent base hydrolysis. The buffer is also used for antigen detection by breaking cross-links between antigens and any substances in its fixation medium. Citrate buffer is a popular choice for immunofluorescent staining applications, as it can aid in stain intensity without contributing to background signal. It is a popular choice for biological research and clinical applications, including pathology, and has had an increasing role in the medical field, as it has been shown to mildly improve dialysis efficiency. It has a shelf life of up to 3 months at room temperature.

Available for purchase here.

To prepare  L of Citrate Buffer (pH 3.0 to 6.2):
Change the value in the textbox above to scale the recipe volume

Current molarity:
0.1 M
Adjust the molarity of the solution by using the slider below
Current pH:
Adjust the pH of the solution by using the slider below

Table 1. Required components
Sodium Citrate dihydrate (mw: 294.10 g/mol)25.703 g0.0874 M
Citric Acid (mw: 192.12 g/mol)2.421 g0.0126 M
  1. Prepare 800 mL of distilled water in a suitable container.
  2. Add 25.703 g of Sodium Citrate dihydrate to the solution.
  3. Add 2.421 g of Citric Acid to the solution.
  4. Adjust solution to final desired pH using HCl or NaOH
  5. Add distilled water until the volume is 1 L.

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Physiological Buffer
pH Buffering
Sample Preparation
Cell/Culture/Growth Media
Gel Electrophoresis


This online tool may be cited as follows


"Quest Calculate™ Citrate Buffer (pH 3.0 to 6.2) Preparation and Recipe." AAT Bioquest, Inc.15 Aug2022


AAT Bioquest, Inc. (2022August 15). Quest Calculate™ Citrate Buffer (pH 3.0 to 6.2) Preparation and Recipe. AAT Bioquest.

This online tool has been cited in 1 publications, including

Nephroprotective and antioxidant effects of ethanol extract of Coprinus comatus mushroom fruit-bodies on streptozotocin-induced diabetic rat models
Journal: (2021): 012078