Calcium Chloride Stock Solution Preparation and Recipe

Calcium chloride is a common calcium salt used in a variety of applications.

To prepare

L of Calcium Chloride Stock Solution:

Change the value in the textbox above to scale the recipe volume

Table 1. Required components

Component	Amount	Concentration
Calcium Chloride (mw: 110.98 g / mol)	#	#

Prepare 800 mL of distilled water in a suitable container.
Add # of Calcium Chloride to the solution
Add distilled water until the volume is 1 L.
Measure the osmolarity using an osmometer: if below 1000 mmol/kg, add more CaCl2; if above 1000, add H2O. (The osmolarity of 1000 mmol/kg is ~0.383 M. Preparation of this calibrated solution is necessary because calcium chloride is hygroscopic, so is not reliably measured by weight. Slight errors in calcium concentration have an outsized effect on synaptic physiology.) Store the solution at 4?C. Solution stored under these conditions appears to be stable for several months.

References

This online resource may be cited as follows

MLA	"Quest Calculate™ Calcium Chloride Stock Solution Preparation and Recipe." AAT Bioquest, Inc., 31 Oct. 2025, https://www.aatbio.com/resources/buffer-preparations-and-recipes/calcium-chloride-stock-solution.
APA	AAT Bioquest, Inc. (2025, October 31). Quest Calculate™ Calcium Chloride Stock Solution Preparation and Recipe. AAT Bioquest. https://www.aatbio.com/resources/buffer-preparations-and-recipes/calcium-chloride-stock-solution.
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