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ACES Buffer (0.1 M, 6.7 pH) Preparation and Recipe

ACES buffer is one of the Good's zwitterionic buffer with a pH range of 6.1-7.2.

To prepare  L of ACES Buffer (0.1 M, 6.7 pH):
Change the value in the textbox above to scale the recipe volume

Table 1. Required components
ACES (mw: 182.20 g/mol)18.22 g0.1 M
  1. Prepare 800 mL of dH2O in a suitable container.
  2. Add 18.22 g of ACES to the solution.
  3. Adjust solution to desired pH by 10N NaOH.
  4. Add dH2O until the volume is 1 L.

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Physiological Buffer
pH Buffering
Sample Preparation
Cell/Culture/Growth Media
Gel Electrophoresis


This online tool may be cited as follows


"Quest Calculate™ ACES Buffer (0.1 M, 6.7 pH) Preparation and Recipe." AAT Bioquest, Inc.26 Jan2023


AAT Bioquest, Inc. (2023January 26). Quest Calculate™ ACES Buffer (0.1 M, 6.7 pH) Preparation and Recipe. AAT Bioquest.