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ACES Buffer (0.1 M, 6.7 pH) Preparation and Recipe
ACES buffer is one of the Good's zwitterionic buffer with a pH range of 6.1-7.2.
To prepare
L of ACES Buffer (0.1 M, 6.7 pH):
Change the value in the textbox above to scale the recipe volume
Table 1. Required components
ComponentAmountConcentration
ACES (mw: 182.2 g / mol)##
  • Prepare 800 mL of dH2O in a suitable container.
  • Add # of ACES to the solution
  • Adjust solution to desired pH by 10N NaOH.
  • Add dH2O until the volume is 1 L.
References
This online resource may be cited as follows
MLA
"Quest Calculate™ ACES Buffer (0.1 M, 6.7 pH) Preparation and Recipe." AAT Bioquest, Inc., 20 Oct. 2025, https://www.aatbio.com/resources/buffer-preparations-and-recipes/aces-buffer-ph-6-7.
APA
AAT Bioquest, Inc. (2025, October 20). Quest Calculate™ ACES Buffer (0.1 M, 6.7 pH) Preparation and Recipe. AAT Bioquest. https://www.aatbio.com/resources/buffer-preparations-and-recipes/aces-buffer-ph-6-7.
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