AAT Bioquest

Ammonium Acetate Preparation and Recipe

Ammonium acetate is widely used in molecular biology in applications such as protein purification and precipitation.

To prepare  L of Ammonium Acetate:
Change the value in the textbox above to scale the recipe volume

Table 1. Required components
ammonium acetate (mw: 77.08 g/mol)770 g10 M
  1. Prepare 800 mL of distilled water in a suitable container.
  2. Add 770 g of ammonium acetate to the solution.
  3. Add distilled water until the volume is 1 L.
  4. Sterilize the solution by passing it through a 0.22-µm filter. Store the solution in tightly sealed bottles at 4°C or at room temperature. Ammonium acetate decomposes in hot H2O and solutions containing it should not be autoclaved.

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This online tool may be cited as follows


"Quest Calculate™ Ammonium Acetate Preparation and Recipe." AAT Bioquest, Inc.23 Jun2024https://www.aatbio.com/resources/buffer-preparations-and-recipes/ammonium-acetate.


AAT Bioquest, Inc. (2024June 23). Quest Calculate™ Ammonium Acetate Preparation and Recipe. AAT Bioquest. https://www.aatbio.com/resources/buffer-preparations-and-recipes/ammonium-acetate.

This online tool has been cited in 1 publications, including

Study On The Adsorption Of Cd+ 2, Cu+ 2, Zn+ 2, CO+ 2, La+ 3 And Y+ 3 Metal Ions On Titanium Oxide-Cerium Phosphate Nanocomposite Membrane
Journal: The Libyan Journal of Science (2022)