Elution Buffer Preparation and Recipe
Elution buffer is commonly used in many applications such as affinity chromatography, immunoprecipitation, protein purification, and DNA extraction to elute proteins or DNA from a ligand or membrane.
To prepare L of Elution Buffer:
Change the value in the textbox above to scale the recipe volume
Change the value in the textbox above to scale the recipe volume
Table 1. Required components
Component | Amount | Concentration |
Sodium chloride (mw: 58.44 g/mol) | 23.38 g | 0.4 M |
- Prepare 800 mL of distilled water in a suitable container.
- Add 23.38 g of Sodium chloride to the solution.
- Add distilled water until the volume is 1 L.
- Filter the solution through a nitrocellulose filter (0.45-?m pore size) and store at room temperature.
Physiological Buffer
pH Buffering
Sample Preparation
BioAssays
Misc
Cell/Culture/Growth Media
Gel Electrophoresis
References
This online tool may be cited as follows
MLA | "Quest Calculate™ Elution Buffer Preparation and Recipe." AAT Bioquest, Inc., 10 Oct. 2024, https://www.aatbio.com/resources/buffer-preparations-and-recipes/elution-buffer. | |
APA | AAT Bioquest, Inc. (2024, October 10). Quest Calculate™ Elution Buffer Preparation and Recipe. AAT Bioquest. https://www.aatbio.com/resources/buffer-preparations-and-recipes/elution-buffer. | |
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