Elution Buffer Preparation and Recipe

Elution buffer is commonly used in many applications such as affinity chromatography, immunoprecipitation, protein purification, and DNA extraction to elute proteins or DNA from a ligand or membrane.

To prepare

L of Elution Buffer:

Change the value in the textbox above to scale the recipe volume

Table 1. Required components

Component	Amount	Concentration
Sodium chloride (mw: 58.44 g / mol)	#	#

Prepare 800 mL of distilled water in a suitable container.
Add # of Sodium chloride to the solution
Add distilled water until the volume is 1 L.
Filter the solution through a nitrocellulose filter (0.45-?m pore size) and store at room temperature.

References

This online resource may be cited as follows

MLA	"Quest Calculate™ Elution Buffer Preparation and Recipe." AAT Bioquest, Inc., 14 Oct. 2025, https://www.aatbio.com/resources/buffer-preparations-and-recipes/elution-buffer.
APA	AAT Bioquest, Inc. (2025, October 14). Quest Calculate™ Elution Buffer Preparation and Recipe. AAT Bioquest. https://www.aatbio.com/resources/buffer-preparations-and-recipes/elution-buffer.
BibTeX	EndNote	RefMan