AAT Bioquest

Elution Buffer Preparation and Recipe

Elution buffer is commonly used in many applications such as affinity chromatography, immunoprecipitation, protein purification, and DNA extraction to elute proteins or DNA from a ligand or membrane.

To prepare  L of Elution Buffer:
Change the value in the textbox above to scale the recipe volume

Table 1. Required components
Sodium chloride (mw: 58.44 g/mol)23.38 g0.4 M
  1. Prepare 800 mL of distilled water in a suitable container.
  2. Add 23.38 g of Sodium chloride to the solution.
  3. Add distilled water until the volume is 1 L.
  4. Filter the solution through a nitrocellulose filter (0.45-?m pore size) and store at room temperature.

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This online tool may be cited as follows


"Quest Calculate™ Elution Buffer Preparation and Recipe." AAT Bioquest, Inc.29 May2024https://www.aatbio.com/resources/buffer-preparations-and-recipes/elution-buffer.


AAT Bioquest, Inc. (2024May 29). Quest Calculate™ Elution Buffer Preparation and Recipe. AAT Bioquest. https://www.aatbio.com/resources/buffer-preparations-and-recipes/elution-buffer.