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Elution Buffer Preparation and Recipe

Elution buffer is commonly used in many applications such as affinity chromatography, immunoprecipitation, protein purification, and DNA extraction to elute proteins or DNA from a ligand or membrane.

To prepare  L of Elution Buffer:
Change the value in the textbox above to scale the recipe volume

Table 1. Required components
Sodium chloride (mw: 58.44 g/mol)23.38 g0.4 M
  1. Prepare 800 mL of distilled water in a suitable container.
  2. Add 23.38 g of Sodium chloride to the solution.
  3. Add distilled water until the volume is 1 L.
  4. Filter the solution through a nitrocellulose filter (0.45-?m pore size) and store at room temperature.

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This online tool may be cited as follows


"Quest Calculate™ Elution Buffer Preparation and Recipe." AAT Bioquest, Inc.6 Dec2022


AAT Bioquest, Inc. (2022December 6). Quest Calculate™ Elution Buffer Preparation and Recipe. AAT Bioquest.