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Elution Buffer Preparation and Recipe
Elution buffer is commonly used in many applications such as affinity chromatography, immunoprecipitation, protein purification, and DNA extraction to elute proteins or DNA from a ligand or membrane.
To prepare
L of Elution Buffer:
Change the value in the textbox above to scale the recipe volume
Table 1. Required components
ComponentAmountConcentration
Sodium chloride (mw: 58.44 g / mol)##
  • Prepare 800 mL of distilled water in a suitable container.
  • Add # of Sodium chloride to the solution
  • Add distilled water until the volume is 1 L.
  • Filter the solution through a nitrocellulose filter (0.45-?m pore size) and store at room temperature.


References
This online resource may be cited as follows
MLA
"Quest CalculateElution Buffer Preparation and Recipe." AAT Bioquest, Inc.14 Oct2025https://www.aatbio.com/resources/buffer-preparations-and-recipes/elution-buffer.
APA
AAT Bioquest, Inc. (2025October 14). Quest CalculateElution Buffer Preparation and Recipe. AAT Bioquest. https://www.aatbio.com/resources/buffer-preparations-and-recipes/elution-buffer.
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