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Buffer Preparation and Recipes


Min pH

Max pH


PBS (Phosphate Buffered Saline) (1X, pH 7.4)7.37.5Common Physiological
HBSS (Hank's Balanced Salt Solution)7.27.6Common Physiological
HHBS (Hank's Buffer with Hepes)7.27.4Common Physiological
Citrate Buffer (pH 3.0 to 6.2)3.06.2Variable pH
Acetate Buffer (pH 3.6 to 5.6)3.75.6Variable pH
Tris Buffer (1 M, pH 7.0)7.07.5Common Physiological
TE Buffer 10X7.58.5Common Physiological
Citrate Buffer (0.1 M, pH 6.0)3.06.2Common Physiological
Acetate Buffer (0.1 M, pH 5.0)3.75.6Common Physiological
Phosphate Buffer (pH 5.8 to 7.4)5.88.0Variable pH
Carbonate-Bicarbonate Buffer (pH 9.2 to 10.6)9.210.8Variable pH
Hydrochloric Acid-Potassium Chloride Buffer (0.1 M, pH 2.0)1.02.2Common Physiological
Glycine-HCl Buffer (0.1 M, pH 3.0)2.23.6Common Physiological
Citrate-Phosphate Buffer (0.15 M, pH 5.0)2.67.6Common Physiological
Glycine-Sodium Hydroxide Buffer (0.08 M, pH 10)8.610.6Common Physiological
EBSS (magnesium, calcium, phenol red) (pH 7.0)7.07.4Common Physiological
Imidazole-HCl Buffer (0.07 M, pH 7.0)6.27.8Common Physiological
Trizma Buffer (pH 7.2 to 9.0)7.29.0Variable pH
MES (0.5 M, pH 6)5.56.7Common Physiological
BES-Buffered Saline (2X) (0.05 M, pH 6.95)6.47.8Common Physiological
MOPS Buffer (10X) (0.2 M, pH 7)6.57.9Common Physiological
TAE Buffer (50X) (0.04 M, pH 8.5)8.08.5Common Physiological
TBS (1 M, pH 7.4)7.27.5Common Physiological
Bicine (1 M, pH 8.26)7.69.0Common Physiological
Tricine (1 M, pH 8.05)7.48.8Common Physiological
TAE (1 M, pH 8.6)8.08.5Common Physiological
CAPS (0.5 M, pH 10.4)9.711.1Common Physiological
HEPPSO (1 M, pH 7.85)7.18.5Common Physiological
CHES (0.5 M, pH 9.5)8.610.0Common Physiological
Potassium Phosphate (pH 5.8 to 8.0)5.88.0Variable pH
Diethanolamine (1 M, pH 9.8)7.89.9Common Physiological
Sodium Borate Buffer (1 M, pH 8.5)8.010.0Common Physiological
Maleic Acid (1 M, pH 7.5)7.08.0Common Physiological