ACK Lysis Buffer Preparation and Recipe

Table 1. Required components

Component	Amount	Concentration
Ammonium chloride (mw: 53.49 g/mol)	8.02 g	0.15 M
Potassium bicarbonate (mw: 100.12 g/mol)	1 g	0.01 M
Disodium EDTA (mw: 372.24 g/mol)	0.0372 g	0.0001 M

1. Prepare 800 mL of distilled water in a suitable container.
2. Add 8.02 g of Ammonium chloride to the solution.
3. Add 1 g of Potassium bicarbonate to the solution.
4. Add 0.0372 g of Disodium EDTA to the solution.
5. Adjust the pH to 7.2-7.4.
6. Add distilled water until the volume is 1 L.
7. Store for up to 6 mo at room temperature.

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