AAT Bioquest

ACK Lysis Buffer Preparation and Recipe

ACK Lysis Buffer is used to lyse red blood cells.

To prepare  L of ACK Lysis Buffer:
Change the value in the textbox above to scale the recipe volume

Table 1. Required components
Ammonium chloride (mw: 53.49 g/mol)8.02 g0.15 M
Potassium bicarbonate (mw: 100.12 g/mol)1 g0.01 M
Disodium EDTA (mw: 372.24 g/mol)0.0372 g0.0001 M
  1. Prepare 800 mL of distilled water in a suitable container.
  2. Add 8.02 g of Ammonium chloride to the solution.
  3. Add 1 g of Potassium bicarbonate to the solution.
  4. Add 0.0372 g of Disodium EDTA to the solution.
  5. Adjust the pH to 7.2-7.4.
  6. Add distilled water until the volume is 1 L.
  7. Store for up to 6 mo at room temperature.

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Physiological Buffer
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This online tool may be cited as follows


"Quest Calculate™ ACK Lysis Buffer Preparation and Recipe." AAT Bioquest, Inc.20 Apr2024https://www.aatbio.com/resources/buffer-preparations-and-recipes/ack-lysis-buffer.


AAT Bioquest, Inc. (2024April 20). Quest Calculate™ ACK Lysis Buffer Preparation and Recipe. AAT Bioquest. https://www.aatbio.com/resources/buffer-preparations-and-recipes/ack-lysis-buffer.