ACK Lysis Buffer Preparation and Recipe

ACK Lysis Buffer is used to lyse red blood cells.

To prepare

L of ACK Lysis Buffer:

Change the value in the textbox above to scale the recipe volume

Table 1. Required components

Component	Amount	Concentration
Ammonium chloride (mw: 53.49 g / mol)	#	#
Potassium bicarbonate (mw: 100.12 g / mol)	#	#
Disodium EDTA (mw: 372.24 g / mol)	#	#

Prepare 800 mL of distilled water in a suitable container.
Add # of Ammonium chloride to the solution
Add # of Potassium bicarbonate to the solution
Add # of Disodium EDTA to the solution
Adjust the pH to 7.2-7.4.
Add distilled water until the volume is 1 L.
Store for up to 6 mo at room temperature.

References

This online resource may be cited as follows

MLA	"Quest Calculate™ ACK Lysis Buffer Preparation and Recipe." AAT Bioquest, Inc., 21 Oct. 2026, https://www.aatbio.com/resources/buffer-preparations-and-recipes/ack-lysis-buffer.
APA	AAT Bioquest, Inc. (2026, October 21). Quest Calculate™ ACK Lysis Buffer Preparation and Recipe. AAT Bioquest. https://www.aatbio.com/resources/buffer-preparations-and-recipes/ack-lysis-buffer.
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This online resource has been cited in 2 publications, including

Increased Plasma Mitochondrial DNA Copy Number and Association with Complications after Coronary Artery Bypass Grafting: Results of a Pilot Cohort Study

Authors:

Kuzheleva, Elena A and Garganeeva, Alla A and Sleptsov, Aleksei A and Tukish, Olga V and Kondratiev, Michail Yu and Syromyatnikova, Ekaterina E and Bogdanov, Yuri I and Baev, Andrey E

Mitochondrial DNA Copy Number Is Increased in Plasma and Associated with Postoperative Complications After Coronary Artery Bypass Grafting

Authors:

Kuzheleva, Elena A and Garganeeva, Alla A and Sleptsov, Alexei A and Tukish, Olga V and Kondratiev, Michail Yu and Syromyatnikova, Ekaterina E and Dordgieva, Beligma B and Bogdanov, Yuri I and Baev, Andrey E