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ACK Lysis Buffer Preparation and Recipe
ACK Lysis Buffer is used to lyse red blood cells.
To prepareL of ACK Lysis Buffer:
Change the value in the textbox above to scale the recipe volume
Table 1. Required components
| Component | Amount | Concentration |
|---|---|---|
| Ammonium chloride (mw: 53.49 g / mol) | # | # |
| Potassium bicarbonate (mw: 100.12 g / mol) | # | # |
| Disodium EDTA (mw: 372.24 g / mol) | # | # |
- Prepare 800 mL of distilled water in a suitable container.
- Add # of Ammonium chloride to the solution
- Add # of Potassium bicarbonate to the solution
- Add # of Disodium EDTA to the solution
- Adjust the pH to 7.2-7.4.
- Add distilled water until the volume is 1 L.
- Store for up to 6 mo at room temperature.
References
This online resource may be cited as follows
MLA | "Quest Calculate™ ACK Lysis Buffer Preparation and Recipe." AAT Bioquest, Inc., 9 Oct. 2025, https://www.aatbio.com/resources/buffer-preparations-and-recipes/ack-lysis-buffer. | |
APA | AAT Bioquest, Inc. (2025, October 9). Quest Calculate™ ACK Lysis Buffer Preparation and Recipe. AAT Bioquest. https://www.aatbio.com/resources/buffer-preparations-and-recipes/ack-lysis-buffer. | |
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