Ethidium Bromide Solution Preparation and Recipe

Ethidium bromide is commonly used nucleic acid stain for gel electrophoresis.

To prepare

L of Ethidium Bromide Solution:

Change the value in the textbox above to scale the recipe volume

Table 1. Required components

Component	Amount	Concentration
Ethidium bromide (mw: 394.31 g / mol)	#	#

Prepare 800 mL of distilled water in a suitable container.
Add # of Ethidium bromide to the solution
Add distilled water until the volume is 1 L.
Stir on a magnetic stirrer for several hours to ensure that the dye has dissolved. Wrap the container in aluminum foil or transfer the 10 mg/mL solution to a dark bottle and store at room temperature.

References

This online resource may be cited as follows

MLA	"Quest Calculate™ Ethidium Bromide Solution Preparation and Recipe." AAT Bioquest, Inc., 6 Nov. 2025, https://www.aatbio.com/resources/buffer-preparations-and-recipes/ethidium-bromide.
APA	AAT Bioquest, Inc. (2025, November 6). Quest Calculate™ Ethidium Bromide Solution Preparation and Recipe. AAT Bioquest. https://www.aatbio.com/resources/buffer-preparations-and-recipes/ethidium-bromide.
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