AAT Bioquest

Ethidium Bromide Solution Preparation and Recipe

Ethidium bromide is commonly used nucleic acid stain for gel electrophoresis.

To prepare  L of Ethidium Bromide Solution:
Change the value in the textbox above to scale the recipe volume

Table 1. Required components
Ethidium bromide (mw: 394.31 g/mol)10 g0.02536 M
  1. Prepare 800 mL of distilled water in a suitable container.
  2. Add 10 g of Ethidium bromide to the solution.
  3. Add distilled water until the volume is 1 L.
  4. Stir on a magnetic stirrer for several hours to ensure that the dye has dissolved. Wrap the container in aluminum foil or transfer the 10 mg/mL solution to a dark bottle and store at room temperature.

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Physiological Buffer
pH Buffering
Sample Preparation
Cell/Culture/Growth Media
Gel Electrophoresis


This online tool may be cited as follows


"Quest Calculate™ Ethidium Bromide Solution Preparation and Recipe." AAT Bioquest, Inc.28 May2024https://www.aatbio.com/resources/buffer-preparations-and-recipes/ethidium-bromide.


AAT Bioquest, Inc. (2024May 28). Quest Calculate™ Ethidium Bromide Solution Preparation and Recipe. AAT Bioquest. https://www.aatbio.com/resources/buffer-preparations-and-recipes/ethidium-bromide.