Glycine (0.1 M, pH 2.2) Preparation and Recipe
Glycine is a bulking agent that can help promote protein stability.
To prepare L of Glycine (0.1 M, pH 2.2):
Change the value in the textbox above to scale the recipe volume
Change the value in the textbox above to scale the recipe volume
Table 1. Required components
Component | Amount | Concentration |
Glycine (Sigma G7126) (mw: 75.07 g/mol) | 7.5 g | 0.0999 M |
- Prepare 800 mL of distilled water in a suitable container.
- Add 7.5 g of Glycine (Sigma G7126) to the solution.
- Adjust the pH to 2.2 with HCl.
- Add distilled water until the volume is 1 L.
- Autoclave.
Physiological Buffer
pH Buffering
Sample Preparation
BioAssays
Misc
Cell/Culture/Growth Media
Gel Electrophoresis
References
This online tool may be cited as follows
MLA | "Quest Calculate™ Glycine (0.1 M, pH 2.2) Preparation and Recipe." AAT Bioquest, Inc., 1 Dec. 2024, https://www.aatbio.com/resources/buffer-preparations-and-recipes/glycine-0-1-m-ph-2-2. | |
APA | AAT Bioquest, Inc. (2024, December 1). Quest Calculate™ Glycine (0.1 M, pH 2.2) Preparation and Recipe. AAT Bioquest. https://www.aatbio.com/resources/buffer-preparations-and-recipes/glycine-0-1-m-ph-2-2. | |
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