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Glycine (0.1 M, pH 2.2) Preparation and Recipe

Glycine is a bulking agent that can help promote protein stability.

To prepare  L of Glycine (0.1 M, pH 2.2):
Change the value in the textbox above to scale the recipe volume

Table 1. Required components
Glycine (Sigma G7126) (mw: 75.07 g/mol)7.5 g0.0999 M
  1. Prepare 800 mL of distilled water in a suitable container.
  2. Add 7.5 g of Glycine (Sigma G7126) to the solution.
  3. Adjust the pH to 2.2 with HCl.
  4. Add distilled water until the volume is 1 L.
  5. Autoclave.

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Physiological Buffer
pH Buffering
Sample Preparation
Cell/Culture/Growth Media
Gel Electrophoresis


This online tool may be cited as follows


"Quest Calculate™ Glycine (0.1 M, pH 2.2) Preparation and Recipe." AAT Bioquest, Inc.3 Feb2023


AAT Bioquest, Inc. (2023February 3). Quest Calculate™ Glycine (0.1 M, pH 2.2) Preparation and Recipe. AAT Bioquest.