AAT Bioquest

PIPES Buffer (1 M, 6.8 pH) Preparation and Recipe

PIPES buffer is one of the Good's zwitterionic buffer with a pH range of 6.1-7.5.

To prepare  L of PIPES Buffer (1 M, 6.8 pH):
Change the value in the textbox above to scale the recipe volume

Table 1. Required components
PIPES (mw: 302.37 g/mol)302.37 g1 M
  1. Prepare 800 mL of dH2O in a suitable container.
  2. Add 302.37 g of PIPES to the solution.
  3. Solubility of PIPES free acid is low in acidic range. Adjust solution to desired pH by 10N NaOH.
  4. Add dH2O until the volume is 1 L.

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Physiological Buffer
pH Buffering
Sample Preparation
Cell/Culture/Growth Media
Gel Electrophoresis


This online tool may be cited as follows


"Quest Calculate™ PIPES Buffer (1 M, 6.8 pH) Preparation and Recipe." AAT Bioquest, Inc.20 Jun2024https://www.aatbio.com/resources/buffer-preparations-and-recipes/pipes-buffer-ph-6-8.


AAT Bioquest, Inc. (2024June 20). Quest Calculate™ PIPES Buffer (1 M, 6.8 pH) Preparation and Recipe. AAT Bioquest. https://www.aatbio.com/resources/buffer-preparations-and-recipes/pipes-buffer-ph-6-8.