PIPES Buffer (1 M, 6.8 pH) Preparation and Recipe

PIPES buffer is one of the Good's zwitterionic buffer with a pH range of 6.1-7.5.

To prepare

L of PIPES Buffer (1 M, 6.8 pH):

Change the value in the textbox above to scale the recipe volume

Table 1. Required components

Component	Amount	Concentration
PIPES (mw: 302.37 g / mol)	#	#

Prepare 800 mL of dH2O in a suitable container.
Add # of PIPES to the solution
Solubility of PIPES free acid is low in acidic range. Adjust solution to desired pH by 10N NaOH.
Add dH2O until the volume is 1 L.

References

This online resource may be cited as follows

MLA	"Quest Calculate™ PIPES Buffer (1 M, 6.8 pH) Preparation and Recipe." AAT Bioquest, Inc., 5 Dec. 2025, https://www.aatbio.com/resources/buffer-preparations-and-recipes/pipes-buffer-ph-6-8.
APA	AAT Bioquest, Inc. (2025, December 5). Quest Calculate™ PIPES Buffer (1 M, 6.8 pH) Preparation and Recipe. AAT Bioquest. https://www.aatbio.com/resources/buffer-preparations-and-recipes/pipes-buffer-ph-6-8.
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