PIPES Buffer (1 M, 6.8 pH) Preparation and Recipe
PIPES buffer is one of the Good's zwitterionic buffer with a pH range of 6.1-7.5.
To prepare L of PIPES Buffer (1 M, 6.8 pH):
Change the value in the textbox above to scale the recipe volume
Change the value in the textbox above to scale the recipe volume
Table 1. Required components
Component | Amount | Concentration |
PIPES (mw: 302.37 g/mol) | 302.37 g | 1 M |
- Prepare 800 mL of dH2O in a suitable container.
- Add 302.37 g of PIPES to the solution.
- Solubility of PIPES free acid is low in acidic range. Adjust solution to desired pH by 10N NaOH.
- Add dH2O until the volume is 1 L.
Physiological Buffer
pH Buffering
Sample Preparation
BioAssays
Misc
Cell/Culture/Growth Media
Gel Electrophoresis
References
This online tool may be cited as follows
MLA | "Quest Calculate™ PIPES Buffer (1 M, 6.8 pH) Preparation and Recipe." AAT Bioquest, Inc., 9 Nov. 2024, https://www.aatbio.com/resources/buffer-preparations-and-recipes/pipes-buffer-ph-6-8. | |
APA | AAT Bioquest, Inc. (2024, November 9). Quest Calculate™ PIPES Buffer (1 M, 6.8 pH) Preparation and Recipe. AAT Bioquest. https://www.aatbio.com/resources/buffer-preparations-and-recipes/pipes-buffer-ph-6-8. | |
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