Tricine (1 M, pH 8.05) Preparation and Recipe

Tricine is derived from the amino acids tris and glycine. It is a dipolar ion (Zwitterionic) and hydroxyl radical scavenger, and is used extensively for SDS-PAGE applications for small proteins. Tricine buffer is also commonly used for electrophoresis procedures.

To prepare

L of Tricine (1 M, pH 8.05):

Change the value in the textbox above to scale the recipe volume

Table 1. Required components

Component	Amount	Concentration
Tricine (mw: 179.17 g / mol)	#	#

Prepare 800 mL of dH2O in a suitable container.
Add # of Tricine to the solution
Adjust solution to desired pH with 10N NaOH.
Add dH2O until the volume is 1 L.

References

This online resource may be cited as follows

MLA	"Quest Calculate™ Tricine (1 M, pH 8.05) Preparation and Recipe." AAT Bioquest, Inc., 29 Sep. 2025, https://www.aatbio.com/resources/buffer-preparations-and-recipes/tricine-ph-8-05.
APA	AAT Bioquest, Inc. (2025, September 29). Quest Calculate™ Tricine (1 M, pH 8.05) Preparation and Recipe. AAT Bioquest. https://www.aatbio.com/resources/buffer-preparations-and-recipes/tricine-ph-8-05.
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