Tricine (1 M, pH 8.05) Preparation and Recipe
Tricine is derived from the amino acids tris and glycine. It is a dipolar ion (Zwitterionic) and hydroxyl radical scavenger, and is used extensively for SDS-PAGE applications for small proteins. Tricine buffer is also commonly used for electrophoresis procedures.
To prepare L of Tricine (1 M, pH 8.05):
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Change the value in the textbox above to scale the recipe volume
Table 1. Required components
Component | Amount | Concentration |
Tricine (mw: 179.17 g/mol) | 179.17 g | 1 M |
- Prepare 800 mL of dH2O in a suitable container.
- Add 179.17 g of Tricine to the solution.
- Adjust solution to desired pH with 10N NaOH.
- Add dH2O until the volume is 1 L.
Physiological Buffer
pH Buffering
Sample Preparation
BioAssays
Misc
Cell/Culture/Growth Media
Gel Electrophoresis
References
This online tool may be cited as follows
MLA | "Quest Calculate™ Tricine (1 M, pH 8.05) Preparation and Recipe." AAT Bioquest, Inc., 11 Nov. 2024, https://www.aatbio.com/resources/buffer-preparations-and-recipes/tricine-ph-8-05. | |
APA | AAT Bioquest, Inc. (2024, November 11). Quest Calculate™ Tricine (1 M, pH 8.05) Preparation and Recipe. AAT Bioquest. https://www.aatbio.com/resources/buffer-preparations-and-recipes/tricine-ph-8-05. | |
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