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Tricine (1 M, pH 8.05) Preparation and Recipe

Tricine is derived from the amino acids tris and glycine. It is a dipolar ion (Zwitterionic) and hydroxyl radical scavenger, and is used extensively for SDS-PAGE applications for small proteins. Tricine buffer is also commonly used for electrophoresis procedures.

To prepare  L of Tricine (1 M, pH 8.05):
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Table 1. Required components
Tricine (mw: 179.17 g/mol)179.17 g1 M
  1. Prepare 800 mL of dH2O in a suitable container.
  2. Add 179.17 g of Tricine to the solution.
  3. Adjust solution to desired pH with 10N NaOH.
  4. Add dH2O until the volume is 1 L.

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Physiological Buffer
pH Buffering
Sample Preparation
Cell/Culture/Growth Media
Gel Electrophoresis


This online tool may be cited as follows


"Quest Calculate™ Tricine (1 M, pH 8.05) Preparation and Recipe." AAT Bioquest, Inc.19 Jun2024https://www.aatbio.com/resources/buffer-preparations-and-recipes/tricine-ph-8-05.


AAT Bioquest, Inc. (2024June 19). Quest Calculate™ Tricine (1 M, pH 8.05) Preparation and Recipe. AAT Bioquest. https://www.aatbio.com/resources/buffer-preparations-and-recipes/tricine-ph-8-05.