AAT Bioquest

What are the limitations of the insect protein expression system?

Posted August 5, 2023


One limitation is time efficiency, as it involves time-consuming steps such as transfection, selection, and virus amplification. These systems typically require longer periods of time to be carried out compared to bacterial systems. Another drawback is that it requires very expensive media, and may be especially costly for small-scale experiments. Additionally, viral infection causes cell lysis in the late stage which leads to the production of immature proteins. Lastly, glycosylation is different from the mammalian system which could result in improper maintenance of epitopes on protein.

Additional resources

A Comparative Analysis of Recombinant Protein Expression in Different Biofactories: Bacteria, Insect Cells and Plant Systems

Antibodies and Proteomics