Ferroptosis is an ROS-dependent form of regulated cell death. Prominent markers of ferroptosis include: 

• ROS and RNS: Reactive oxygen species (ROS) and reactive nitrogen species (RNS) play significant roles in regulating cell survival and death and are markers of ferroptosis 
• Lipid peroxidation: Lipid peroxidation is a predominant marker of ferroptosis. The oxidative degradation of lipids, leading to production of cytotoxic substances such as reactive aldehydes, lipid free radicals, and lipid hydroperoxides, eventually causes cell damage and death. 
• Overexpression of certain genes and proteins: The overexpression of specific genes and proteins is considered a biomarker of ferroptosis.
• Prostaglandin-Endoperoxide Synthase 2 (PTGS2):PTGS2, a vital enzyme in prostaglandin biosynthesis, is considered a biomarker of ferroptosis but not a driver. PTGS2 encodes cyclooxygenase-2 (COX-2) and acts as a dioxygenase and peroxidase. 
• Acyl-CoA Synthetase Long-Chain Family Member 4 (ACSL4):ACSL4 alters the composition of cellular lipids, influencing ferroptosis sensitivity. It also acts as both - a specific biomarker and driver of ferroptosis.
• Transferrin Receptor 1 (TFR1):A carrier protein, TFR1 is responsible for causing iron overload by transporting Fe3+ from the extracellular space to the intracellular space. TFR1 accumulation on the cell surface is a feature and marker of ferroptosis.